1887

Abstract

shows extraordinary tolerance to DNA damage, and exhibits differential gene expression and protein recycling. A putative response regulator, the DRB0091 (RadR) ORF, was identified from a pool of DNA-binding proteins induced in response to gamma radiation in this bacterium. is located upstream of , which encodes a putative sensor histidine kinase (RadS) on the megaplasmid. Deletion of these genes both individually and together resulted in hypersensitivity to DNA-damaging agents and a delayed or altered double-strand break repair. A Δ double mutant and a Δ single mutant showed nearly identical responses to gamma radiation and UVC. Wild-type RadR and RadS complemented the corresponding mutant strains, but also exhibited significant cross-complementation, albeit at lower doses of gamma radiation. The transcript was not detected in the Δ mutant, suggesting the existence of a operon. Recombinant RadS was autophosphorylated and could catalyse the transfer of γ phosphate from ATP to RadR . These results indicated the functional interaction of RadS and RadR, and suggested a role for the RadS/RadR two-component system in the radiation resistance of this bacterium.

Funding
This study was supported by the:
  • , Bhabha Atomic Research Centre
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2011-10-01
2020-11-29
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