1887

Abstract

moves in response to environmental chemical cues using a chemotaxis two-component signal-transduction system. Autoinducer-2 (AI-2) is a quorum-sensing signal produced by the LuxS protein that accumulates in the bacterial environment in a density-dependent manner. We showed previously that a mutant was defective in motility on soft agar plates. Here we report that deletion of the gene resulted in swimming behaviour with a reduced frequency of stops as compared to the wild-type strain. Stopping frequency was restored to wild-type levels by genetic complementation of the mutation or by addition of synthetic 4,5-dihydroxy-2,3-pentanedione (DPD), which cyclizes to form AI-2. Synthetic DPD also increased the frequency of stops in wild-type , similar to the behaviour induced by the known chemorepellent HCl. We found that whereas mutants lacking the chemoreceptor genes or responded to an exogenous source of synthetic DPD, the chemoreceptor mutant was non-responsive to a gradient or uniform distribution of the chemical. Furthermore, a double mutant lacking both and exhibited chemotactic behaviour similar to the single mutant, whereas a double mutant lacking both and the chemotransduction gene behaved like a nonchemotactic single mutant, supporting the model that functions in a signalling pathway downstream of and upstream of . We conclude that perceives LuxS-produced AI-2 as a chemorepellent via the chemoreceptor TlpB.

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2011-09-01
2019-10-15
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