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Volume 157, Issue 7

The flavanone naringenin reduces the production of quorum sensing-controlled virulence factors in PAO1 Free

Abstract

Preliminary screening of the Malagasy plant for compounds attenuating the production of quorum sensing (QS)-controlled virulence factors in bacteria led to the identification of active fractions containing flavonoids. In the present study, several flavonoids belonging to the flavone, flavanone, flavonol and chalcone structural groups were screened for their capacity to reduce the production of QS-controlled factors in the opportunistic pathogen (strain PAO1). Flavanones (i.e. naringenin, eriodictyol and taxifolin) significantly reduced the production of pyocyanin and elastase in without affecting bacterial growth. Consistently, naringenin and taxifolin reduced the expression of several QS-controlled genes (i.e. , , , , , , and ) in PAO1. Naringenin also dramatically reduced the production of the acylhomoserine lactones -(3-oxododecanoyl)--homoserine lactone (3-oxo-C12-HSL) and -butanoyl--homoserine lactone (C4-HSL), which is driven by the and gene products, respectively. In addition, using mutant strains deficient for autoinduction (Δ and Δ) and LasR- and RhlR-based biosensors, it was shown that QS inhibition by naringenin not only is the consequence of a reduced production of autoinduction compounds but also results from a defect in the proper functioning of the RlhR–C4-HSL complex. Widely distributed in the plant kingdom, flavonoids are known for their numerous and determinant roles in plant physiology, plant development and in the success of plant–rhizobia interactions, but, as shown here, some of them also have a role as inhibitors of the virulence of pathogenic bacteria by interfering with QS mechanisms.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Funding
This study was supported by the:
  • F.R.S.-F.N.R.S.
  • Fonds de la Recherche Scientifique, Belgium
  • A.U.F.
  • Agence Universitaire de la Francophonie, Madagascar - Belgium
  • C.U.D.
  • Commission Universitaire pour le Développement, Belgium
  • Fondation David et Alice Van Buuren (Belgium)
  • C.U.D.
  • C.U.I. Université de Ouagadougou, Burkina Faso
© 2011 SGM
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2011-07-01
2024-04-25
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The flavanone naringenin reduces the production of quorum sensing-controlled virulence factors in Pseudomonas aeruginosa PAO1
Microbiology 157, 2120 (2011); https://doi.org/10.1099/mic.0.049338-0
/content/journal/micro/10.1099/mic.0.049338-0
/content/journal/micro/10.1099/mic.0.049338-0
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