1887

Abstract

Bacteria encode multiple protein secretion systems that are crucial for interaction with the environment and with hosts. In recent years, attention has focused on type VI secretion systems (T6SSs), which are specialized transporters widely encoded in Proteobacteria. The myriad of processes associated with these secretion systems could be explained by subclasses of T6SS, each involved in specialized functions. To assess diversity and predict function associated with different T6SSs, comparative genomic analysis of 34 genomes was performed. This identified 70 T6SSs, with at least one locus in every strain, except for A1501. By comparing 11 core genes of the T6SS, it was possible to identify five main phylogenetic clusters, with strains typically carrying T6SSs from more than one clade. In addition, most strains encode additional and genes, which encode extracellular structural components of the secretion apparatus. Using a combination of phylogenetic and meta-analysis of transcriptome datasets it was possible to associate specific subsets of VgrG and Hcp proteins with each T6SS clade. Moreover, a closer examination of the genomic context of genes in multiple strains highlights a number of additional genes associated with these regions. It is proposed that these genes may play a role in secretion or alternatively could be new T6S effectors.

Funding
This study was supported by the:
  • Science Foundation of Ireland (Award 09/RFP/BMT2350, 08/RFP/GEN1319, 08/RFP/GEN1295 and 07/IN.1/B948)
  • Department of Agriculture, Fisheries and Food (Award 08RDC629, 06RDC506, 06RDC459, 06-377 and 06-321)
  • European Commission (Award Marie Curie TOK:TRAMWAYS and MTKD-CT2006-042062)
  • ERCSET (Award 05/EDIV/FP107/INTERPAM)
  • Marine Institute Beaufort (Award C&CRA 2007/082)
  • HRB (Award HRA/2009/146, RP/2007/290 and RP/2006/271)
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2011-06-01
2021-07-27
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