1887

Abstract

In the extremely thermophilic bacterium HB8, one of the four TetR-family transcriptional regulators, which we named . FadR, negatively regulated the expression of several genes, including those involved in fatty acid degradation, both and . . FadR repressed the expression of the target genes by binding pseudopalindromic sequences covering the predicted −10 hexamers of their promoters, and medium-to-long straight-chain (C10–18) fatty acyl-CoA molecules were effective for transcriptional derepression. An X-ray crystal structure analysis revealed that . FadR bound one lauroyl (C12)-CoA molecule per FadR monomer, with its acyl chain moiety in the centre of the FadR molecule, enclosed within a tunnel-like substrate-binding pocket surrounded by hydrophobic residues, and the CoA moiety interacting with basic residues on the protein surface. The growth of . HB8, with palmitic acid as the sole carbon source, increased the expression of FadR-regulated genes. These results indicate that in . HB8, medium-to-long straight-chain fatty acids can be used for metabolic energy under the control of FadR, although the major fatty acids found in this strain are - and -branched-chain (C15 and 17) fatty acids.

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2011-06-01
2020-01-21
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