1887

Abstract

Two regulators, Aur1P and Aur1R, have been previously found to control expression of the polyketide gene cluster involved in biosynthesis of the angucycline-like antibiotic auricin in CCM 3239 in a cascade mechanism. Here, we describe the characterization of two additional regulatory genes, and , encoding homologues of the SARP family of transcriptional activators that were identified in the upstream part of the cluster. Expression of both genes is directed by a single promoter, and , respectively, induced in late exponential phase. Disruption of in CCM 3239 had no effect on auricin production. However, the disruption of dramatically reduced auricin compared with its parental wild-type strain. Transcription from the promoter, directing expression of the first biosynthetic gene in the auricin gene cluster, was similarly decreased in the CCM 3239 mutant. Transcription from the promoter increased in the CCM 3239 mutant strain, and the TetR family negative regulator Aur1R was shown to specifically bind the promoter. These results indicate a complex regulation of the auricin cluster by the additional SARP family transcriptional activator Aur1PR3.

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2011-06-01
2020-10-24
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