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Volume 157, Issue 6

Cell-surface nucleolin is sequestered into EPEC microcolonies and may play a role during infection Open Access

Abstract

Nucleolin is a prominent nucleolar protein that is mobilized into the cytoplasm during infection by enteropathogenic (EPEC). Nucleolin also exists at low levels at the cell surface of eukaryotic cells and here we show that upon infection of an intestinal cell model, EPEC recruits and subsequently sequesters cell-surface EGFP-nucleolin into extracellularly located bacterial microcolonies. The recruitment of nucleolin was evident around bacteria within the centre of the microcolonies that were not directly associated with actin-based pedestals. Incubation of host intestinal cells with different ligands that specifically bind nucleolin impaired the ability of EPEC to disrupt epithelial barrier function but did not inhibit bacterial attachment or other effector-driven processes such as pedestal formation or microvilli effacement. Taken together, this work suggests that EPEC exploits two spatially distinct pools of nucleolin during the infection process.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Funding
This study was supported by the:
  • Wellcome Trust
© 2011 SGM
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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2011-06-01
2024-04-25
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Cell-surface nucleolin is sequestered into EPEC microcolonies and may play a role during infection
Microbiology 157, 1761 (2011); https://doi.org/10.1099/mic.0.047506-0
/content/journal/micro/10.1099/mic.0.047506-0
/content/journal/micro/10.1099/mic.0.047506-0
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