1887

Abstract

is a significant human pathogen worldwide and is responsible for severe nosocomial and community-acquired infections. Although enterococcal meningitis is rare, mortality is considerable, reaching 21 %. Nevertheless, the pathogenetic mechanisms of this infection remain poorly understood, even though the ability of to avoid or survive phagocytic attack may be very important during the infection process. We previously showed that the manganese-cofactored superoxide dismutase (MnSOD) SodA of was implicated in oxidative stress responses and, interestingly, in the survival within mouse peritoneal macrophages using an infection model. In the present study, we investigated the role of MnSOD in the interaction of with microglia, the brain-resident macrophages. By using an infection model, murine microglial cells were challenged in parallel with the wild-type strain JH2-2 and its isogenic deletion mutant. While both strains were phagocytosed by microglia efficiently and to a similar extent, the Δ mutant was found to be significantly more susceptible to microglial killing than JH2-2, as assessed by the antimicrobial protection assay. In addition, a significantly higher percentage of acidic Δ-containing phagosomes was found and these also underwent enhanced maturation as determined by the expression of endolysosomal markers. In conclusion, these results show that the MnSOD of contributes to survival of the bacterium in microglial cells by influencing their antimicrobial activity, and this could even be important for intracellular killing in neutrophils and thus for pathogenesis.

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2011-06-01
2020-01-23
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