1887

Abstract

The role of the HU nucleoid-associated proteins in gene regulation was examined in serovar Typhimurium. The dimeric HU protein consists of different combinations of its and subunits. Transcriptomic analysis was performed with cultures growing at 37 °C at 1, 4 and 6 h after inoculation with mutants that lack combinations of HU and HU . Distinct but overlapping patterns of gene expression were detected at each time point for each of the three mutants, revealing not one but three regulons of genes controlled by the HU proteins. Mutations in the genes altered the expression of regulatory and structural genes in both the SPI1 and SPI2 pathogenicity islands. The double mutant was defective in invasion of epithelial cell lines and in its ability to survive in macrophages. The double mutant also had defective swarming activity and a competitive fitness disadvantage compared with the wild-type. In contrast, inactivation of just the gene resulted in increased fitness and correlated with the upregulation of members of the RpoS regulon in exponential-phase cultures. Our data show that HU coordinates the expression of genes involved in central metabolism and virulence and contributes to the success of as a pathogen.

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2011-04-01
2019-10-19
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Effects of mutations on genes involved in respiration. Expression of the operon in the mutants. Expression of ABC transporter genes in the mutants. Detection of RpoS by Western blotting in the mutants. Effect of the mutations on gene expression. Reporter plasmid DNA supercoiling in the mutants. Oligonucleotide primers used in this study. All supplementary figures and Supplementary Table S1 are available as a single PDF(344 KB).

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Genes showing at least a twofold increase ( Table S2) or decrease ( Table S3) in expression in the mutant at the 1, 4 or 6 h time points.

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Genes showing at least a twofold increase ( Table S2) or decrease ( Table S3) in expression in the mutant at the 1, 4 or 6 h time points.

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Genes showing at least a twofold increase ( Table S4) or decrease ( Table S5) in expression in the mutant at the 1, 4 or 6 h time points.

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Genes showing at least a twofold increase ( Table S4) or decrease ( Table S5) in expression in the mutant at the 1, 4 or 6 h time points.

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Genes showing at least a twofold increase ( Table S6) or decrease ( Table S7) in expression in the mutant at the 1, 4 or 6 h time points.

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Genes showing at least a twofold increase ( Table S6) or decrease ( Table S7) in expression in the mutant at the 1, 4 or 6 h time points.

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