1887

Abstract

, the causal agent of wheat head blight, shows intrinsic resistance to amine fungicides. It is commonly accepted that the amines target sterol C-14 reductase and sterol Δ–Δ isomerase of ergosterol biosynthesis, encoded by the genes and , respectively. Analysis of the genome sequence of revealed that the fungus contains two paralogous genes ( and ), which are homologous to the of . In this study, we disrupted and in . Compared to the wild-type strain HN9-1, and deletion mutants did not show recognizable phenotypic changes in mycelial growth on potato dextrose agar or in virulence on wheat heads. HPLC analysis showed that the amount of ergosterol in or deletion mutants was not significantly different from that in the wild-type strain. These results indicate that neither of the two genes is essential for growth, pathogenicity or ergosterol biosynthesis in . deletion mutants exhibited significantly increased sensitivity to amine fungicides, including tridemorph, fenpropidin and spiroxamine, but not to non-amine fungicides. In contrast, deletion mutants did not show changed sensitivity to any amine tested. The resistance of the deletion mutant to amines was restored by genetic complementation of the mutant with wild-type . These results indicate that controls the intrinsic resistance of to amines. The finding of this study provides new insights into amine resistance in filamentous fungi.

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2011-06-01
2019-10-21
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Spectrophotometric analysis of sterol intermediates in the parent strain HN9-1, gene deletion mutant (Δerg24A-1), and gene deletion mutant (Δerg24B-2) [ PDF] (39 kb) Phylogenetic tree generated by using the neighbour-joining method with MEGA 4.1 software on the basis of deduced amino acid sequences of FgErg2 (FGSG_07315.3) from strain HN9-1 (boxed), and those from (AfErg2, GenBank accession no. XP_750221.1), (AnErg2, XP_658055.1), (BcErg2, XP_001558454), (CaErg2, EEQ43057.1), (CgErg2, XP_449233.1), (FoErg2, FOXG_01590), (FvErg2, FVEG_07949), (MoErg2, XP_363040.1), (NcErg2, XP_961312.2), (ScErg2, AAS56448.1), (SpErg2, NP_593324.1) and (SsErg2, XP_001592497.1) [ PDF] (62 kb)

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Spectrophotometric analysis of sterol intermediates in the parent strain HN9-1, gene deletion mutant (Δerg24A-1), and gene deletion mutant (Δerg24B-2) [ PDF] (39 kb) Phylogenetic tree generated by using the neighbour-joining method with MEGA 4.1 software on the basis of deduced amino acid sequences of FgErg2 (FGSG_07315.3) from strain HN9-1 (boxed), and those from (AfErg2, GenBank accession no. XP_750221.1), (AnErg2, XP_658055.1), (BcErg2, XP_001558454), (CaErg2, EEQ43057.1), (CgErg2, XP_449233.1), (FoErg2, FOXG_01590), (FvErg2, FVEG_07949), (MoErg2, XP_363040.1), (NcErg2, XP_961312.2), (ScErg2, AAS56448.1), (SpErg2, NP_593324.1) and (SsErg2, XP_001592497.1) [ PDF] (62 kb)

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