1887

Abstract

Adaptation to environmental stress is an important survival characteristic of any bacterial species. As a soil-dwelling saprophyte, is exposed to factors such as UV light and rounds of freezing and thawing that occur in temperate climates. Numerous studies in have linked histone-like proteins to stress resistance and adaptation. We hypothesized that the ‘histone-like’ protein Hlp might likewise be involved in the stress response of . The gene was inactivated and the Δ strain was found to be more susceptible to UV light and to the stress created by repeated cycles of freezing and thawing. In addition, loss of Hlp altered the colony morphology and allowed the organism to grow dispersed in the absence of a detergent, suggesting changes in the cell wall composition. As cell wall changes could affect permeability to certain antibiotics, the susceptibility of Δ to kanamycin, rifamipicin, ethambutol and isoniazid (INH) was tested. Δ was more susceptible to INH, but loss of Hlp did not affect susceptibility to the other antibiotics tested. This suggests that the increased sensitivity of Δ to INH was unlikely to be the result of alterations in cell permeability.

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2011-02-01
2020-01-21
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