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Abstract

The transcriptional activation response relies on a repertoire of transcriptional activators, which decipher regulatory information through their specific binding to cognate sequences, and their capacity to selectively recruit the components that constitute a given transcriptional complex. We have addressed the possibility of achieving novel transcriptional responses by the construction of a new transcriptional regulator – the Hap2-3-5-Gln3 hybrid modulator – harbouring the HAP complex polypeptides that constitute the DNA-binding domain (Hap2-3-5) and the Gln3 activation domain, which usually act in an uncombined fashion. The results presented in this paper show that transcriptional activation of and under repressive nitrogen conditions is achieved through the action of the novel Hap2-3-5-Gln3 transcriptional regulator. We propose that the combination of the Hap DNA-binding and Gln3 activation domains results in a hybrid modulator that elicits a novel transcriptional response not evoked when these modulators act independently.

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2011-03-01
2019-12-13
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vol. , part 3, pp. 879 - 889

( PDF, 83 kb): Hap2- , Hap4- and Gln3- activate transcription The amount of Gln3-Myc13 is equivalent in and Δ- strains Multiple alignments of and intergenic sequences of species reveal conserved elements that could function as regulatory elements Multiple alignments of and intergenic sequences of species reveal that the promoter does not carry HAP-binding sites



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