1887

Abstract

Most members of the phylum Firmicutes harbour a two-component system (TCS), LiaSR, which is involved in the response to cell envelope stress elicited most notably by inhibitors of the lipid II cycle. In all LiaSR systems studied in detail, LiaSR-mediated signal transduction has been shown to be negatively controlled by a membrane protein, LiaF, encoded upstream of . In this study we have analysed the LiaSR orthologue of (LiaSR). Whole-genome transcriptional profiling indicated that activation of LiaSR results in a remodelling of the cell envelope via the massive upregulation of membrane-associated and extracytoplasmic proteins in the presence of inducing stimuli. As shown for other LiaSR TCSs, LiaSR is activated by cell wall-active antibiotics. We demonstrate that the level of phosphorylated LiaR, which is required for the induction of the LiaSR regulon, is controlled by the interplay between the histidine kinase and phosphatase activities of the bifunctional sensor protein LiaS. Our data suggest that the phosphatase activity of LiaS is stimulated by LiaF in the absence of cell envelope stress.

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2011-02-01
2019-12-10
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