1887

Abstract

pv. () is the causative agent of citrus canker. This bacterium develops a characteristic biofilm on both biotic and abiotic surfaces. To evaluate the participation of the single flagellum of in biofilm formation, mutants in the (flagellin) and the (hook) genes were generated. Swimming motility, assessed on 0.25 % agar plates, was markedly reduced in and mutants. However, the and mutants exhibited a flagellar-independent surface translocation on 0.5 % agar plates. Mutation of either the or the gene, which both encode proteins involved in cell–cell signalling mediated by diffusible signal factor (DSF), led to a reduction in both flagellar-dependent and flagellar-independent surface translocation, indicating a regulatory role for DSF in both types of motility. Confocal laser scanning microscopy of biofilms produced in static culture demonstrated that the flagellum is also involved in the formation of mushroom-shaped structures and water channels, and in the dispersion of biofilms. The presence of the flagellum was required for mature biofilm development on lemon leaf surfaces. The absence of flagellin produced a slight reduction in pathogenicity and this reduction was more severe when the complete flagellum structure was absent.

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2011-03-01
2020-01-18
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[ PDF, 74 kb]: Generation of and mutants of and phenotypic characterization Swimming motility in the (a) and (b) complemented mutants as measured in 0.25% NYGB agar plates after 72 h at 28 °C



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