1887

Abstract

Transcription of genes coding for formate dehydrogenases ( genes) and hydrogenases ( genes) in and was studied following growth under different conditions. Under all conditions tested, all and genes were transcribed. However, transcription levels of the individual genes varied depending on the substrate and growth conditions. Our results strongly suggest that in syntrophically grown cells, the [FeFe]-hydrogenase (encoded by Sfum_844-46), FDH1 (Sfum_2703-06) and Hox (Sfum_2713-16) may confurcate electrons from NADH and ferredoxin to protons and carbon dioxide to produce hydrogen and formate, respectively. Based on bioinformatic analysis, a membrane-integrated energy-converting [NiFe]-hydrogenase (Mhun_1741-46) of might be involved in the energy-dependent reduction of CO to formylmethanofuran. The best candidates for F-dependent , -methyl-H MPT and , ,-methylene-HMPT reduction are the cytoplasmic [NiFe]-hydrogenase and FDH1. 16S rRNA ratios indicate that in one of the triplicate co-cultures of and , less energy was available for This led to enhanced transcription of genes coding for the Rnf-complex (Sfum_2694-99) and of several and genes. The Rnf-complex probably reoxidized NADH with ferredoxin reduction, followed by ferredoxin oxidation by the induced formate dehydrogenases and hydrogenases.

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2011-01-01
2020-11-26
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