1887

Abstract

The genetic diversity of three temperate fruit tree phytoplasmas ‘ Phytoplasma prunorum’, ‘ P. mali’ and ‘ P. pyri’ has been established by multilocus sequence analysis. Among the four genetic loci used, the genes and distinguished 30 and 24 genotypes, respectively, and showed the highest variability. Percentage of substitution for ranged from 50 to 68 % according to species. Percentage of substitution varied between 9 and 12 % for , whereas it was between 5 and 6 % for and . In the case of ‘ P. prunorum’ the three most prevalent genotypes were detected in both plants and insect vectors, confirming that the prevalent isolates are propagated by insects. The four isolates known to be hypo-virulent had the same sequence, indicating a possible monophyletic origin. Haplotype network reconstructed by eBURST revealed that among the 34 haplotypes of ‘ P. prunorum’, the four hypo-virulent isolates also grouped together in the same clade. Genotyping of some Spanish and Azerbaijanese ‘ P. pyri’ isolates showed that they shared some alleles with ‘ P. prunorum’, supporting for the first time to our knowledge, the existence of inter-species recombination between these two species.

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2011-02-01
2019-12-07
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