1887

Abstract

Currently, the species is divided into four subspecies, subsp. , subsp. , subsp. and subsp. These classifications were based mainly on phenotypic identification methods and few studies have used genotypic identification methods. As a result, these subspecies have not yet been reliably delineated. In this study, the four subspecies of were discriminated by phenotype and by genotypic identification [amplified-fragment length polymorphism (AFLP) and multilocus sequence typing (MLST)] methods. The MLST method developed here was based on the analysis of seven housekeeping genes (, , , , , and ). The MLST method had good discriminatory ability: the 41 strains of examined were divided into 34 sequence types, with 29 sequence types represented by only a single strain. The sequence types were divided into eight groups. These groups could be discriminated as representing different subspecies. The results of the AFLP and MLST analyses were consistent. The type strain of subsp. , YIT 0080, was clearly discriminated from the other strains currently classified as members of this subspecies, which were located close to strains of subsp. s. The MLST scheme developed in this study should be a useful tool for the identification of strains of to the subspecies level.

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2011-03-01
2020-07-14
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