1887

Abstract

Forty fluorescent strains isolated from white and red cocoyam roots were tested for their ability to synthesize -acyl--homoserine lactones (acyl-HSLs). Remarkably, only isolates from the red cocoyam rhizosphere that were antagonistic against the cocoyam root rot pathogen and synthesized phenazine antibiotics produced acyl-HSLs. This supports the assumption that acyl-HSL production is related to the antagonistic activity of the strains. After detection, the signal molecules were identified through TLC-overlay and liquid chromatography-multiple MS (LC-MS/MS) analysis. In our representative strain, CMR12a, production of the signal molecules could be assigned to two quorum-sensing (QS) systems. The first one is the QS system for phenazine production, PhzI/PhzR, which seemed to be well conserved, since it was genetically organized in the same way as in the well-described phenazine-producing strains 2-79, PCL1391 and 30-84. The newly characterized genes and make up the second QS system of CMR12a, under the control of the uncommon -3-hydroxy-dodecanoyl-homoserine lactone (3-OH-C12-HSL) and with low similarity to other QS systems. No clear function could yet be assigned to the CmrI/CmrR system, although it contributes to the biocontrol capability of CMR12a. Both the PhzI/PhzR and CmrI/CmrR systems are controlled by the GacS/GacA two-component regulatory system.

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2011-02-01
2020-07-07
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