1887

Abstract

The putative phosphoporin encoded by of O1 is expressed during infection and is essential for the intestinal colonization of infant mice. , its expression is induced under inorganic phosphate (P) limitation in a PhoB/R-dependent manner. In this work we demonstrated that VCA1008 has a strain-specific role in the physiology and pathogenicity of O1. Disruption of led to a growth defect, an inability to colonize and a high susceptibility to sodium deoxycholate (DOC; the major bile compound) in the El Tor biotype strain N16961, but did not affect the classical strain O395 in the same way. Furthermore, promoter activity was higher in N16961 cells grown under a low P supply in the presence of DOC than in the absence of the detergent. In the P-limited cells, was positively regulated by PhoB, but when DOC was added to the medium, it negatively affected the PhoB-mediated activation of the gene, and enhanced expression in a ToxR-dependent manner. These findings reveal for the first time a complex strain-specific interplay between ToxR and PhoB/R systems to control porin genes, as well as the influence of DOC on the expression of PhoB- and ToxR-regulated genes and pathogenesis in pandemic strains of . .

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2010-10-01
2020-01-24
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