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Volume 156, Issue 10

An insert in the gene distinguishes a pharyngeal and a blood isolate of found in the same individual Free

Abstract

Expression of the extensive arsenal of virulence factors by is controlled by many regulators, of which CovRS is one of the best characterized and can influence ∼15 % of the genome. Animal models have established that mutants of arise spontaneously resulting in highly invasive organisms. We analysed a pharyngeal and a blood isolate of recovered from the same individual 13 days apart. The two isolates varied in many phenotypic properties including SpeB production, which were reflected in transcriptomic analyses. PFGE, multilocus sequence typing and partial sequencing of some key genes failed to show any differences except for an 11 bp insert in the gene in the blood isolate which caused a premature termination of transcription. Complementation of a fully functional gene into the blood isolate resulted in high expression of CovS and expression of . These results, showing a pharyngeal and a blood isolate from a single individual differing by a simple insertion, provide evidence for the model that regulatory gene mutations allow to invade different niches in the body.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): FBS, fetal bovine serum , GAS, group A streptococci , MLST, multilocus sequence typing , qRT-PCR, quantitative RT-PCR and THY, Todd–Hewitt broth supplemented with 0.1 % yeast extract
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2010-10-01
2024-04-19
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An insert in the covS gene distinguishes a pharyngeal and a blood isolate of Streptococcus pyogenes found in the same individual
Microbiology 156, 3085 (2010); https://doi.org/10.1099/mic.0.042614-0
/content/journal/micro/10.1099/mic.0.042614-0
/content/journal/micro/10.1099/mic.0.042614-0
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