1887

Abstract

Expression of the extensive arsenal of virulence factors by is controlled by many regulators, of which CovRS is one of the best characterized and can influence ∼15 % of the genome. Animal models have established that mutants of arise spontaneously resulting in highly invasive organisms. We analysed a pharyngeal and a blood isolate of recovered from the same individual 13 days apart. The two isolates varied in many phenotypic properties including SpeB production, which were reflected in transcriptomic analyses. PFGE, multilocus sequence typing and partial sequencing of some key genes failed to show any differences except for an 11 bp insert in the gene in the blood isolate which caused a premature termination of transcription. Complementation of a fully functional gene into the blood isolate resulted in high expression of CovS and expression of . These results, showing a pharyngeal and a blood isolate from a single individual differing by a simple insertion, provide evidence for the model that regulatory gene mutations allow to invade different niches in the body.

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2010-10-01
2024-11-06
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References

  1. Beres S. B., Richter E. W., Nagiec M. J., Sumby P., Porcella S. F., DeLeo F. R., Musser J. M. 2006; Molecular genetic anatomy of inter- and intraserotype variation in the human bacterial pathogen group A Streptococcus. Proc Natl Acad Sci U S A 103:7059–7064
    [Google Scholar]
  2. Churchward G. 2007; The two faces of Janus: virulence gene regulation by CovR/S in group A streptococci. Mol Microbiol 64:34–41
    [Google Scholar]
  3. Cole J. N., McArthur J. D., McKay F. C., Sanderson-Smith M. L., Cork A. J., Ranson M., Rohde M., Itzek A., Sun H. other authors 2006; Trigger for group A streptococcal M1T1 invasive disease. FASEB J 20:1745–1747
    [Google Scholar]
  4. Cunningham M. W. 2000; Pathogenesis of group A streptococcal infections. Clin Microbiol Rev 13:470–511
    [Google Scholar]
  5. Cunningham M. W. 2008; Pathogenesis of group A streptococcal infections and their sequelae. Adv Exp Med Biol 609:29–42
    [Google Scholar]
  6. Dalton T. L., Scott J. R. 2004; CovS inactivates CovR and is required for growth under conditions of general stress in Streptococcus pyogenes. J Bacteriol 186:3928–3937
    [Google Scholar]
  7. Engleberg N. C., Heath A., Miller A., Rivera C., DiRita V. J. 2001; Spontaneous mutations in the CsrRS two-component regulatory system of Streptococcus pyogenes result in enhanced virulence in a murine model of skin and soft tissue infection. J Infect Dis 183:1043–1054
    [Google Scholar]
  8. Erdem G., Ford J., Johnson D., Abe L., Yamaga K., Kaplan E. 2005; Erythromycin-resistant group A streptococcal isolates collected between 2000 and 2005 in Oahu, Hawaii, and their emm types. J Clin Microbiol 43:2497–2499
    [Google Scholar]
  9. Erdem G., Mizumoto C., Esaki D., Abe L., Yamaga K., Reddy V., Effler P. 2009; Streptococcal emm types in Hawaii: a region with high incidence of acute rheumatic fever. Pediatr Infect Dis J 28:13–16
    [Google Scholar]
  10. Federle M. J., McIver K. S., Scott J. R. 1999; A response regulator that represses transcription of several virulence operons in the group A streptococcus. J Bacteriol 181:3649–3657
    [Google Scholar]
  11. Froeliger E. H., Fives-Taylor P. 1998; Analysis of adherence-associated gene expression in Streptococcus parasangusis: a method for RNA isolation. Methods Cell Sci 20:143–151
    [Google Scholar]
  12. Graham M. R., Smoot L. M., Migliaccio C. A., Virtaneva K., Sturdevant D. E., Porcella S. F., Federle M. J., Adams G. J., Scott J. R., Musser J. M. 2002; Virulence control in group A Streptococcus by a two-component gene regulatory system: global expression profiling and in vivo infection modeling. Proc Natl Acad Sci U S A 99:13855–13860
    [Google Scholar]
  13. Hagman M. M., Dale J. B., Stevens D. L. 1999; Comparison of adherence to and penetration of a human laryngeal epithelial cell line by group A streptococci of various M protein types. FEMS Immunol Med Microbiol 23:195–204
    [Google Scholar]
  14. Heath A., DiRita V. J., Barg N. L., Engleberg N. C. 1999; A two-component regulatory system, CsrR–CsrS, represses expression of three Streptococcus pyogenes virulence factors, hyaluronic acid capsule, streptolysin S, and pyrogenic exotoxin B. Infect Immun 67:5298–5305
    [Google Scholar]
  15. Levin J. C., Wessels M. R. 1998; Identification of csrR/ csrS, a genetic locus that regulates hyaluronic acid capsule synthesis in group A Streptococcus. Mol Microbiol 30:209–219
    [Google Scholar]
  16. Musser J. M., DeLeo F. R. 2005; Toward a genome-wide systems biology analysis of host–pathogen interactions in group A Streptococcus. Am J Pathol 167:1461–1472
    [Google Scholar]
  17. Nagamune H., Ohkura K., Ohkuni H. 2005; Molecular basis of group A streptococcal pyrogenic exotoxin B. J Infect Chemother 11:1–8
    [Google Scholar]
  18. North M. 1994; Cysteine endopeptidases of parasitic protozoa. In Methods in Enzymology pp 523–539 Edited by Barrett A. San Diego: Academic Press;
    [Google Scholar]
  19. Ravins M., Jaffe J., Hanski E., Shetzigovski I., Natanson-Yaron S., Moses A. E. 2000; Characterization of a mouse-passaged, highly encapsulated variant of group A streptococcus in in vitro and in vivo studies. J Infect Dis 182:1702–1711
    [Google Scholar]
  20. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  21. Simon D., Ferretti J. J. 1991; Electrotransformation of Streptococcus pyogenes with plasmid and linear DNA. FEMS Microbiol Lett 66:219–224
    [Google Scholar]
  22. Sumby P., Whitney A. R., Graviss E. A., DeLeo F. R., Musser J. M. 2006; Genome-wide analysis of group A streptococci reveals a mutation that modulates global phenotype and disease specificity. PLoS Pathog 2:e5
    [Google Scholar]
  23. Svensson M. D., Scaramuzzino D. A., Sjobring U., Olsen A., Frank C., Bessen D. E. 2000; Role for a secreted cysteine proteinase in the establishment of host tissue tropism by group A streptococci. Mol Microbiol 38:242–253
    [Google Scholar]
  24. Tart A. H., Walker M. J., Musser J. M. 2007; New understanding of the group A Streptococcus pathogenesis cycle. Trends Microbiol 15:318–325
    [Google Scholar]
  25. Virtaneva K., Graham M. R., Porcella S. F., Hoe N. P., Su H., Graviss E. A., Gardner T. J., Allison J. E., Lemon W. J. other authors 2003; Group A Streptococcus gene expression in humans and cynomolgus macaques with acute pharyngitis. Infect Immun 71:2199–2207
    [Google Scholar]
  26. Virtaneva K., Porcella S. F., Graham M. R., Ireland R. M., Johnson C. A., Ricklefs S. M., Babar I., Parkins L. D., Romero R. A. other authors 2005; Longitudinal analysis of the group A Streptococcus transcriptome in experimental pharyngitis in cynomolgus macaques. Proc Natl Acad Sci U S A 102:9014–9019
    [Google Scholar]
  27. Walker M. J., Hollands A., Sanderson-Smith M. L., Cole J. N., Kirk J. K., Henningham A., McArthur J. D., Dinkla K., Aziz R. K. other authors 2007; DNase Sda1 provides selection pressure for a switch to invasive group A streptococcal infection. Nat Med 13:981–985
    [Google Scholar]
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