1887

Abstract

Here, we transcriptionally and phenotypically characterized the gene from . Northern blot analysis identified a monocistronic mRNA strongly induced at 48 and 50 °C. analysis identified that the gene encodes a protein of 868 aa with a predicted molecular mass of approximately 98 kDa, presenting two conserved ATP-binding domains. Sequence analysis also identified a CtsR-binding box upstream of the putative −10 sequence, and inactivation of the gene resulted in an approximately 2-log increase in mRNA expression, confirming ClpB as a member of the CtsR regulon. While expression of was induced by heat stress, a Δ strain grew relatively well under many different stressful conditions, including elevated temperatures. However, expression of ClpB appears to play a major role in induced thermotolerance and in pathogenesis, as assessed by using the virulence model.

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2011-03-01
2020-07-07
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