1887

Abstract

Heptose-containing oligosaccharides (OSs) are found in the outer core of the lipopolysaccharide (LPS) of a subset of non-typable (NT) strains. Candidate genes for the addition of either ----heptose (-Hep) or ----heptose (-Hep) and subsequent hexose sugars to these OSs have been identified from the recently completed genome sequences available for NT strains. and are two sets of related genes in which has homology to genes encoding glycosyltransferases and to genes encoding heptosyltransferases. Each set of genes is variably present across NT strains and is located in a region of the genome with an alternative gene organization between strains that contributes to LPS heterogeneity. Dependent upon the strain background, the LPS phenotype, structure and serum resistance of strains mutated in these genes were altered when compared with the relevant parent strain. Our studies confirm that and usually encode -heptosyl- and -heptosyl transferases, respectively, and that encode glycosyltransferases that play a role in OS extensions of NT LPS.

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2010-11-01
2019-10-18
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