1887

Abstract

The presence, distribution and expression of cassette chromosome recombinase () genes, which are homologous to the staphylococcal genes and are designated genes, were examined in enterococcal isolates (=421) representing 13 different species. A total of 118 (28 %) isolates were positive for genes by PCR, and a number of these were confirmed by Southern hybridization with a probe (=76) and partial DNA sequencing of and genes (=38). genes were present in (58/216, 27 %), (31/38, 82 %), (27/52, 50 %), (1/4, 25 %) and (1/2, 50 %). In the eight other species tested, including (=94), genes were not found. Thirty-eight sequenced genes from five different enterococcal species showed 94–100 % nucleotide sequence identity and linkage PCRs showed heterogeneity in the flanking chromosomal genes. Expression analysis of genes from the DO strain showed constitutive expression as a bicistronic mRNA. The mRNA levels were lower during log phase than stationary phase in relation to total mRNA. Multilocus sequence typing was performed on 39 isolates. genes were detected in both hospital-related (10/29, 34 %) and non-hospital (4/10, 40 %) strains of . Various sequence types were represented by both positive and negative isolates, suggesting acquisition or loss of in . In summary, genes, potentially involved in genome plasticity, are expressed in and are widely distributed in the and species groups.

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2010-12-01
2020-04-04
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