%0 Journal Article %A Thepparit, Chutima %A Bourchookarn, Apichai %A Petchampai, Natthida %A Barker, Steven A. %A Macaluso, Kevin R. %T Interaction of Rickettsia felis with histone H2B facilitates the infection of a tick cell line %D 2010 %J Microbiology, %V 156 %N 9 %P 2855-2863 %@ 1465-2080 %R https://doi.org/10.1099/mic.0.041400-0 %K CpB, carboxypeptidase B %K Co-IP, co-immunoprecipitation %K PPIase, peptidyl-prolyl cis-trans isomerise %K qPCR, quantitative real-time PCR %K RNAi, RNA-mediated interference %I Microbiology Society, %X Haematophagous arthropods are the primary vectors in the transmission of Rickettsia, yet the molecular mechanisms mediating the rickettsial infection of arthropods remain elusive. This study utilized a biotinylated protein pull-down assay together with LC-MS/MS to identify interaction between Ixodes scapularis histone H2B and Rickettsia felis. Co-immunoprecipitation of histone with rickettsial cell lysate demonstrated the association of H2B with R. felis proteins, including outer-membrane protein B (OmpB), a major rickettsial adhesin molecule. The rickettsial infection of tick ISE6 cells was reduced by approximately 25 % via RNA-mediated H2B-depletion or enzymic treatment of histones. The interaction of H2B with the rickettsial adhesin OmpB suggests a role for H2B in mediating R. felis internalization into ISE6 cells. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.041400-0