1887

Abstract

Haematophagous arthropods are the primary vectors in the transmission of , yet the molecular mechanisms mediating the rickettsial infection of arthropods remain elusive. This study utilized a biotinylated protein pull-down assay together with LC-MS/MS to identify interaction between histone H2B and . Co-immunoprecipitation of histone with rickettsial cell lysate demonstrated the association of H2B with proteins, including outer-membrane protein B (OmpB), a major rickettsial adhesin molecule. The rickettsial infection of tick ISE6 cells was reduced by approximately 25 % via RNA-mediated H2B-depletion or enzymic treatment of histones. The interaction of H2B with the rickettsial adhesin OmpB suggests a role for H2B in mediating internalization into ISE6 cells.

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2010-09-01
2019-11-21
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vol. , part 9, pp. 2855 - 2863

affinity pull-down assay. To assess the specificity of the assay, was used in place of Rickettsia. Western blotting analysis of eluates from pull-down assays using biotinylated ISE6 surface proteins and/or . Biotinylated ISE6 cells in 0.1% NP-40/PBS buffer were incubated with or without . bound protein pellets were collected, washed, and eluted using 0.1% NP-40/PBS with 1 M NaCl. Eluates were analysed on Bis-Tris gels with Western blotting analysis using peroxidase-conjugated streptavidin. A banding pattern distinct from ISE6/ was observed.



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