1887

Abstract

Acetohydroxyacid synthase (AHAS) is a biosynthetic enzyme essential for synthesis of branched-chain amino acids. The genome sequence of revealed genes encoding four catalytic subunits, (), (), () and (), and one regulatory subunit, (), of AHAS. All these genes were found to be expressed in growing . Each AHAS subunit gene was cloned and expressed in . AHAS activity of IlvB1 and IlvG was found in cell-free lysates and with recombinant purified proteins. Kinetic studies with purified IlvG revealed positive cooperativity towards substrate and cofactors. To understand the role of the catalytic subunits in the biology of , expression of AHAS genes was analysed in different physiological conditions. , and were differentially expressed. The role of in persistence is known, but the upregulation of and in extended stationary phase, , and in acid stress and hypoxic environments, suggests the relevance of AHAS enzymes in the metabolism and survival of by functioning as catabolic AHAS. These enzymes are therefore potential targets for drug development.

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2011-01-01
2020-07-07
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