1887

Abstract

The operon was sequenced in 42 representative isolates of (32), (6) and (4). A total of 27 and 20 alleles were identified whilst the operon was represented by 28 unique alleles that could be assigned to seven classes. There were 1566 (34.8 % variation) polymorphic nucleotide sites and 482 (32.1 % variation) variable inferred amino acid positions among the 42 sequences. The operons of serotype A2 isolates are, with one exception, substantially more diverse than those of the other serotypes and most likely have a different ancestral origin. The phylogeny has been severely disrupted by numerous small- and large-scale intragenic recombination events. In addition, assortative (entire gene) recombination events, involving either the entire operon or the individual and genes, have played a major role in shaping structure and it's distribution in the three species. Our findings indicate that a common gene pool exists for in , and . In particular, , and ovine isolates share a large portion of the gene, and this probably reflects selection for a conserved TbpA protein that provides effective iron uptake in sheep. Bovine and ovine serotype A2 lineages have very different alleles. Bovine-like alleles have been partially, or completely, replaced by ovine-like alleles in ovine serotype A2 isolates, suggesting that different transferrin receptors are required by serotype A2 isolates for optimum iron uptake in cattle and sheep. Conversely, the alleles of bovine-pathogenic serotype A1 and A6 isolates are very similar to those of closely related ovine isolates, suggesting a recent and common evolutionary origin.

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2011-01-01
2020-01-27
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