1887

Abstract

Larvae of , the greater wax moth, provide an alternative infection model for many human pathogens as they are amenable to use at elevated incubation temperatures (37 °C). This study and a parallel study by Mukherjee [Mukherjee, K., Altincicek, B., Hain, T., Domann, E., Vilcinskas, A. & Chakraborty, T. (2010). , 310–317] establish this insect host as an appropriate model to investigate the pathogenesis of species. In this study we show that inoculation with initiates a dynamic infection in and that production of the cytolysin listeriolysin O (LLO) is necessary for toxicity and bacterial growth. Production of LLO by the non-pathogenic species is sufficient to induce mortality in the insect model. We employed real-time bioluminescence imaging to examine the dynamics of listerial growth and virulence gene expression in the model. Analysis of promoter fusions demonstrated significant induction of virulence gene expression upon introduction of the pathogen into insects at both 30 and 37 °C. The host response to listerial infection was examined which demonstrated that haemocyte destruction accompanies pathogenesis and is preceded by activation of the phenoloxidase system. Furthermore, we demonstrate that is pathogenic to through a persistence mechanism that implicates an alternative mechanism for pathogenicity in this model.

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2010-11-01
2019-10-14
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