1887

Abstract

Biomaterial-associated infections are the major cause of implant failure and can develop many years after implantation. Success or failure of an implant depends on the balance between host tissue integration and bacterial colonization. Here, we describe a new model for the post-operative bacterial contamination of implant surfaces and investigate the effects of contamination on the balance between mammalian cell growth and bacterial biofilm formation. U2OS osteosarcoma cells were seeded on poly(methyl methacrylate) in different densities and allowed to grow for 24 h in a parallel-plate flow chamber at a low shear rate (0.14 s), followed by contamination with ATCC 35983 at a shear rate of 11 s. The U2OS cells and staphylococci were allowed to grow simultaneously for another 24 h under low-shear conditions (0.14 s). Mammalian cell growth was severely impaired when the bacteria were introduced to surfaces with a low initial cell density (2.5×10 cells cm), but in the presence of higher initial cell densities (8.2×10 cells cm and 17×10 cells cm), contaminating staphylococci did not affect cell growth. This study is believed to be the first to show that a critical coverage by mammalian cells is needed to effectively protect a biomaterial implant against contaminating bacteria.

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2010-10-01
2020-01-22
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References

  1. Christensen, G. D., Baldassarri, L. & Simpson, W. A. ( 1995; ). Methods for studying microbial colonization of plastics. Methods Enzymol 253, 477–500.
    [Google Scholar]
  2. Dexter, S. J., Pearson, R. G., Davies, M. C., Camara, M. & Shakesheff, K. M. ( 2001; ). A comparison of the adhesion of mammalian cells and Staphylococcus epidermidis on fibronectin-modified polymer surfaces. J Biomed Mater Res 56, 222–227.[CrossRef]
    [Google Scholar]
  3. Fitzgerald, R. H. ( 1979; ). Microbiologic environment of the conventional operating-room. Arch Surg 114, 772–775.[CrossRef]
    [Google Scholar]
  4. Gristina, A. G. ( 1987; ). Biomaterial-centered infection: microbial adhesion versus tissue integration. Science 237, 1588–1595.[CrossRef]
    [Google Scholar]
  5. Gristina, A. G. ( 1994; ). Implant failure and the immune-incompetent fibro-inflammatory zone. Clin Orthop Relat Res 298, 106–118.
    [Google Scholar]
  6. Gristina, A. G., Rovere, G. D., Shoji, H. & Nicastro, J. F. ( 1976; ). An in vitro study of bacterial response to inert and reactive metals and to methyl methacrylate. J Biomed Mater Res 10, 273–281.[CrossRef]
    [Google Scholar]
  7. Gristina, A. G., Naylor, P. & Myrvik, Q. ( 1988; ). Infections from biomaterials and implants: a race for the surface. Med Prog Technol 14, 205–224.
    [Google Scholar]
  8. Gristina, A. G., Naylor, P. T. & Myrvik, Q. N. ( 1990; ). Musculoskeletal infection, microbial adhesion and antibiotic resistance. Infect Dis Clin North Am 4, 391–408.
    [Google Scholar]
  9. Habash, M. & Reid, G. ( 1999; ). Microbial biofilms: their development and significance for medical device-related infections. J Clin Pharmacol 39, 887–898.[CrossRef]
    [Google Scholar]
  10. Kadurugamuwa, J. L., Sin, L., Albert, E., Yu, J., Francis, K., DeBoer, M., Rubin, M., Kawahara, C. B., Parr, T. R. & Contag, P. R. ( 2003; ). Direct continuous method for monitoring biofilm infection in a mouse model. Infect Immun 71, 882–890.[CrossRef]
    [Google Scholar]
  11. Lydon, M. J., Minett, T. W. & Tighe, B. J. ( 1985; ). Cellular interactions with synthetic-polymer surfaces in culture. Biomaterials 6, 396–402.[CrossRef]
    [Google Scholar]
  12. Massey, R. C., Horsburgh, M. J., Lina, G., Hook, M. & Recker, M. ( 2006; ). Opinion - The evolution and maintenance of virulence in Staphylococcus aureus: a role for host-to-host transmission? Nat Rev Microbiol 4, 953–958.[CrossRef]
    [Google Scholar]
  13. Ohara-Nemoto, Y., Haraga, H., Kimura, S. & Nemoto, T. K. ( 2008; ). Occurrence of staphylococci in the oral cavities of healthy adults and nasal–oral trafficking of the bacteria. J Med Microbiol 57, 95–99.[CrossRef]
    [Google Scholar]
  14. Okell, C. C. & Elliott, C. D. ( 1935; ). Bacteriaemia and oral sepsis with special reference to the etiology of subacute endocarditis. Lancet 2, 869–875.
    [Google Scholar]
  15. Proctor, R. A., Von Eiff, C., Kahl, B. C., Becker, K., McNamara, P., Herrmann, M. & Peters, G. ( 2006; ). Small colony variants: a pathogenic form of bacteria that facilitates persistent and recurrent infections. Nat Rev Microbiol 4, 295–305.[CrossRef]
    [Google Scholar]
  16. Singh, R., Ray, P., Das, A. & Sharma, M. ( 2009; ). Role of persisters and small-colony variants in antibiotic resistance of planktonic and biofilm-associated Staphylococcus aureus: an in vitro study. J Med Microbiol 58, 1067–1073.[CrossRef]
    [Google Scholar]
  17. Subbiahdoss, G., Kuijer, R., Grijpma, D. W., Van der Mei, H. C. & Busscher, H. J. ( 2009; ). Microbial biofilm growth vs. tissue integration: “the race for the surface” experimentally studied. Acta Biomater 5, 1399–1404.[CrossRef]
    [Google Scholar]
  18. Subbiahdoss, G., Grijpma, D. W., Van der Mei, H. C., Busscher, H. J. & Kuijer, R. ( 2010; ). Microbial biofilm growth vs. tissue integration on biomaterials with different wettabilities and a polymer–brush coating. J Biomed Mater Res A 9, 533–538.
    [Google Scholar]
  19. van Wachem, P. B., Beugeling, T., Feijen, J., Bantjes, A., Detmers, J. P. & Vanaken, W. G. ( 1985; ). Interaction of cultured human endothelial cells with polymeric surfaces of different wettabilities. Biomaterials 6, 403–408.[CrossRef]
    [Google Scholar]
  20. van Wachem, P. B., Hogt, A. H., Beugeling, T., Feijen, J., Bantjes, A., Detmers, J. P. & Vanaken, W. G. ( 1987; ). Adhesion of cultured human endothelial cells onto methacrylate polymers with varying surface wettability and charge. Biomaterials 8, 323–328.[CrossRef]
    [Google Scholar]
  21. Vuong, C. & Otto, M. ( 2002; ). Staphylococcus epidermidis infections. Microbes Infect 4, 481–489.[CrossRef]
    [Google Scholar]
  22. Young, E. J. & Sugarman, B. ( 1988; ). Infections in prosthetic devices. Surg Clin North Am 68, 167–180.
    [Google Scholar]
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