1887

Abstract

Transferrin (Tf) is a host glycoprotein capable of binding two ferric-iron ions to become holotransferrin (holoTf), which transports iron in to all cells. is a parasitic protozoan able to use holoTf as a sole iron source . The mechanism by which this parasite scavenges iron from holoTf is unknown. An holoTf-binding protein (Tfbp) was purified by using an anti-human transferrin receptor (TfR) monoclonal antibody. Tfbp was identified by MS/MS analysis and database searches as acetaldehyde/alcohol dehydrogenase-2 (ADH2), an iron-dependent enzyme. Both Tfbp and ADH2 bound holoTf and were recognized by the anti-human TfR antibody, indicating that they correspond to the same protein. It was found that the amoebae internalized holoTf through clathrin-coated pits, suggesting that holoTf endocytosis could be important for the parasite during colonization and invasion of the intestinal mucosa and liver.

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2011-01-01
2020-05-27
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