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Volume 157, Issue 1

Acetaldehyde/alcohol dehydrogenase-2 (ADH2) and clathrin are involved in internalization of human transferrin by Free

Abstract

Transferrin (Tf) is a host glycoprotein capable of binding two ferric-iron ions to become holotransferrin (holoTf), which transports iron in to all cells. is a parasitic protozoan able to use holoTf as a sole iron source . The mechanism by which this parasite scavenges iron from holoTf is unknown. An holoTf-binding protein (Tfbp) was purified by using an anti-human transferrin receptor (TfR) monoclonal antibody. Tfbp was identified by MS/MS analysis and database searches as acetaldehyde/alcohol dehydrogenase-2 (ADH2), an iron-dependent enzyme. Both Tfbp and ADH2 bound holoTf and were recognized by the anti-human TfR antibody, indicating that they correspond to the same protein. It was found that the amoebae internalized holoTf through clathrin-coated pits, suggesting that holoTf endocytosis could be important for the parasite during colonization and invasion of the intestinal mucosa and liver.

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Keyword(s): Ab, antibody , ECM, extracellular matrix , EhADH2, Entamoeba histolytica acetaldehyde/alcohol dehydrogenase-2 , EhTfbp, Entamoeba histolytica holoTf-binding protein , GAPDH, glyceraldehyde-3-phosphate dehydrogenase , holoTf, holotransferrin , HRP, horseradish peroxidase , Lf, lactoferrin , Tf, transferrin , TfR, transferrin receptor and TRITC, tetramethylrhodamine isothiocyanate
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2011-01-01
2023-12-01
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Acetaldehyde/alcohol dehydrogenase-2 (EhADH2) and clathrin are involved in internalization of human transferrin by Entamoeba histolytica
Microbiology 157, 209 (2011); https://doi.org/10.1099/mic.0.040063-0
/content/journal/micro/10.1099/mic.0.040063-0
/content/journal/micro/10.1099/mic.0.040063-0
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