1887

Abstract

The (or ) fimbrial operon is ubiquitous and conserved in , but its functions remain poorly described. In routine growth media newborn meningitis isolates of . express the meningitis-associated and temperature-regulated (Mat) fimbria, also termed . common pilus (ECP), at 20 °C, and here we show that the six-gene ()-encoded Mat fimbria is needed for temperature-dependent biofilm formation on abiotic surfaces. The deletion mutant of meningitis . IHE 3034 was defective in an early stage of biofilm development and consequently unable to establish a detectable biofilm, contrasting with IHE 3034 derivatives deleted for flagella, type 1 fimbriae or S-fimbriae, which retained the wild-type biofilm phenotype. Furthermore, induced production of Mat fimbriae from expression plasmids enabled biofilm-deficient . K-12 cells to form biofilm at 20 °C. No biofilm was detected with IHE 3034 or MG1655 strains grown at 37 °C. The surface expression of Mat fimbriae and the frequency of Mat-positive cells in the IHE 3034 population from 20 °C were high and remained unaltered during the transition from planktonic to biofilm growth and within the matured biofilm community. Considering the prevalence of the highly conserved locus in . genomes, we hypothesize that Mat fimbria-mediated biofilm formation is an ancestral characteristic of . .

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2010-08-01
2020-07-09
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