1887

Abstract

Current understanding of resistance and susceptibility to vulvovaginal candidiasis challenges existing paradigms of host defence against fungal infection. While abiotic biofilm formation has a clearly established role during systemic infections, it is not known whether forms biofilms on the vaginal mucosa and the possible role of biofilms in disease. and murine vaginitis models were employed to examine biofilm formation by scanning electron and confocal microscopy. strains included 3153A (lab strain), DAY185 (parental control strain), and mutants defective in morphogenesis and/or biofilm formation (/ and /). Both 3153A and DAY815 formed biofilms on the vaginal mucosa and as indicated by high fungal burden and microscopic analysis demonstrating typical biofilm architecture and presence of extracellular matrix (ECM) co-localized with the presence of fungi. In contrast, / and / mutant strains exhibited weak or no biofilm formation/ECM production in both models compared to wild-type strains and complemented mutants despite comparable colonization levels. These data show for the first time that forms biofilms on vaginal epithelium, and that biotic biofilm formation requires regulators of biofilm formation () and morphogenesis ().

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2010-12-01
2019-12-08
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