1887

Abstract

HpdR, an IclR-family regulator in , is a substrate-dependent repressor for the tyrosine catabolic gene . In this study, S1 nuclease protection assays revealed that is subject to a negative autoregulation. Purified HpdR showed specific DNA-binding activity for the promoter region of , indicating that the autoregulation of is performed directly. The disruption of led to reduced production of CDA by J1501, suggesting a positive effect of on CDA biosynthesis. Electrophoretic mobility shift assays showed that HpdR specifically bound to the promoter region of (SCO3229 in the CDA gene cluster), encoding 4-hydroxymandelic acid synthase. Disruption of in J1501 abolished CDA production. It is possible that regulates CDA biosynthesis by controlling the transcription of .

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2010-09-01
2020-10-01
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