1887

Abstract

is a bacterium that resides in the normal human gastro-intestinal tract; however, it is also the most commonly isolated Gram-negative obligate anaerobe from human clinical infections, such as intra-abdominal abscesses, and the most common cause of anaerobic bacteraemia. Abscess formation is important in bacterial containment, limiting dissemination of infection and bacteraemia. In this study, we investigated binding and degradation of human fibrinogen, the major structural component involved in fibrin abscess formation. We have shown that NCTC9343 binds human fibrinogen. A putative fibrinogen-binding protein, designated BF-FBP, identified in the genome sequence of NCTC9343, was cloned and expressed in . The purified recombinant BF-FBP bound primarily to the human fibrinogen B-chain. In addition, we have identified fibrinogenolytic activity in exponential phase culture supernatants, associated with fibrinogenolytic metalloproteases in NCTC9343 and 638R, and cysteine protease activity in YCH46. All nine clinical isolates of examined degraded human fibrinogen; with eight isolates, initial A-chain degradation was observed, with varying B-chain and -chain degradation. With one blood culture isolate, B-chain and -chain degradation occurred first, followed by subsequent A-chain degradation. Our data raise the possibility that the fibrinogen-binding protein of , along with a variety of fibrinogenolytic proteases, may be an important virulence factor that facilitates dissemination of infection via reduction or inhibition of abscess formation.

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2010-08-01
2020-01-26
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