1887

Abstract

Recombinant VimA protein can interact with the gingipains and several other proteins that may play a role in its biogenesis in . analysis of PG2096, a hypothetical protein that was shown to interact with VimA, suggests that it may have environmental stress resistance properties. To further evaluate the role(s) of PG2096, the predicted open reading frame was PCR amplified from W83 and insertionally inactivated using the antibiotic-resistance cassette. One randomly chosen -defective mutant created by allelic exchange and designated FLL205 was further characterized. Under normal growth conditions at 37 °C, Arg-X and Lys-X gingipain activities in FLL205 were reduced by approximately 35 % and 21 %, respectively, compared to the wild-type strain. However, during prolonged growth at an elevated temperature of 42 °C, Arg-X activity was increased by more than 40 % in FLL205 in comparison to the wild-type strain. In addition, the -defective mutant was more resistant to oxidative stress when treated with 0.25 mM hydrogen peroxide. Taken together these results suggest that the gene, designated (gulator of ingipain activity at elevated emperatures), may be involved in regulating gingipain activity at elevated temperatures and be important in oxidative stress resistance in .

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2010-10-01
2020-01-28
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Phylogenetic analysis of PG2096 ( ) of in relation to and the gene of (neighbour-joining-UPGMA method) [ PDF] (11 kb) Phylogenetic analysis of the amino acid sequence of the G proteins along with the G proteins of other bacteria (Rcn, ; Era, ; Sgp, ) (neighbour-joining-UPGMA method) [ PDF] (12 kb) Multiple sequence alignment of the amino acid sequences of RegT showing conserved domains in relation to the G protein sequences from and other bacteria [ PDF] (500 kb) RT-PCR analysis of gingipain transcripts at different temperatures, showing no change in the expression level [ PDF] (18 kb)

PDF

Phylogenetic analysis of PG2096 ( ) of in relation to and the gene of (neighbour-joining-UPGMA method) [ PDF] (11 kb) Phylogenetic analysis of the amino acid sequence of the G proteins along with the G proteins of other bacteria (Rcn, ; Era, ; Sgp, ) (neighbour-joining-UPGMA method) [ PDF] (12 kb) Multiple sequence alignment of the amino acid sequences of RegT showing conserved domains in relation to the G protein sequences from and other bacteria [ PDF] (500 kb) RT-PCR analysis of gingipain transcripts at different temperatures, showing no change in the expression level [ PDF] (18 kb)

PDF

Phylogenetic analysis of PG2096 ( ) of in relation to and the gene of (neighbour-joining-UPGMA method) [ PDF] (11 kb) Phylogenetic analysis of the amino acid sequence of the G proteins along with the G proteins of other bacteria (Rcn, ; Era, ; Sgp, ) (neighbour-joining-UPGMA method) [ PDF] (12 kb) Multiple sequence alignment of the amino acid sequences of RegT showing conserved domains in relation to the G protein sequences from and other bacteria [ PDF] (500 kb) RT-PCR analysis of gingipain transcripts at different temperatures, showing no change in the expression level [ PDF] (18 kb)

PDF

Phylogenetic analysis of PG2096 ( ) of in relation to and the gene of (neighbour-joining-UPGMA method) [ PDF] (11 kb) Phylogenetic analysis of the amino acid sequence of the G proteins along with the G proteins of other bacteria (Rcn, ; Era, ; Sgp, ) (neighbour-joining-UPGMA method) [ PDF] (12 kb) Multiple sequence alignment of the amino acid sequences of RegT showing conserved domains in relation to the G protein sequences from and other bacteria [ PDF] (500 kb) RT-PCR analysis of gingipain transcripts at different temperatures, showing no change in the expression level [ PDF] (18 kb)

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