1887

Abstract

The unicellular diazotrophic cyanobacterium sp. ATCC 51142 ( 51142) is able to grow aerobically under nitrogen-fixing conditions with alternating light–dark cycles or continuous illumination. This study investigated the effects of carbon and nitrogen sources on 51142 metabolism via C-assisted metabolite analysis and biochemical measurements. Under continuous light (50 μmol photons m s) and nitrogen-fixing conditions, we found that glycerol addition promoted aerobic biomass growth (by twofold) and nitrogenase-dependent hydrogen production [up to 25 μmol H (mg chlorophyll) h], but strongly reduced phototrophic CO utilization. Under nitrogen-sufficient conditions, 51142 was able to metabolize glycerol photoheterotrophically, and the activity of light-dependent reactions (e.g. oxygen evolution) was not significantly reduced. In contrast, sp. PCC 6803 showed apparent mixotrophic metabolism under similar growth conditions. Isotopomer analysis also detected that 51142 was able to fix CO via anaplerotic pathways, and to take up glucose and pyruvate for mixotrophic biomass synthesis.

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2010-08-01
2019-12-09
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