1887

Abstract

The emergence of multi-drug-resistant strains of emphasizes the need to develop new antibiotics. The unique and essential role of the peptide deformylase (PDF) in catalysing the removal of the N-terminal formyl group from newly synthesized polypeptides in eubacteria makes it an attractive antibacterial drug target. In the present study, both deformylase homologues from (SePDF-1 and SePDF-2) were cloned and expressed, and their enzymic activities were characterized. Co-substituted SePDF-1 exhibited much higher enzymic activity ( / 6.3×10 M s) than those of Ni- and Zn-substituted SePDF-1, and SePDF-1 showed much weaker binding ability towards Ni than towards Co and Zn, which is different from PDF in (SaPDF), although they share 80 % amino-acid sequence identity. The determined crystal structure of SePDF-1 was similar to that of (SaPDF), except for differences in the metal-binding sites. The other deformylase homologue, SePDF-2, was shown to have no peptide deformylase activity; the function of SePDF-2 needs to be further investigated.

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2010-10-01
2020-08-05
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