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Volume 156, Issue 9

K-12 YfgF is an anaerobic cyclic di-GMP phosphodiesterase with roles in cell surface remodelling and the oxidative stress response Free

Abstract

The K-12 gene encodes a protein with domains associated with cyclic di-GMP signalling: GGDEF (associated with diguanylate cyclase activity) and EAL (associated with cyclic di-GMP phosphodiesterase activity). Here, it is shown that is expressed under anaerobic conditions from a class II FNR (regulator of fumarate and nitrate reduction)-dependent promoter. Anaerobic expression of is greatest in stationary phase, and in cultures grown at 28 °C, suggesting that low growth rates promote expression. Mutation of resulted in altered cell surface properties and enhanced sensitivity when anaerobic cultures were exposed to peroxides. The purified YfgF GGDEF-EAL (YfgF) and EAL (YfgF) domains possessed cyclic di-GMP-specific phosphodiesterase activity, but lacked diguanylate cyclase activity. However, the catalytically inactive GGDEF domain was required for YfgF dimerization and enhanced cyclic di-GMP phosphodiesterase activity in the presence of physiological concentrations of Mg. The cyclic di-GMP phosphodiesterase activity of YfgF and YfgF was inhibited by the product of the reaction, 5′-phosphoguanylyl-(3′–5′)-guanosine (pGpG). Thus, it is shown that the gene encodes an anaerobic cyclic di-GMP phosphodiesterase that is involved in remodelling the cell surface of K-12 and in the response to peroxide shock, with implications for integrating three global regulatory networks, i.e. oxygen regulation, cyclic di-GMP signalling and the oxidative stress response.

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Keyword(s): EMSA, electrophoretic mobility shift assay , FNR, regulator of fumarate and nitrate reduction , pGpG, 5′-phosphoguanylyl-(3′–5′)-guanosine and RACE, random amplification of cDNA ends
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2010-09-01
2024-04-20
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Escherichia coli K-12 YfgF is an anaerobic cyclic di-GMP phosphodiesterase with roles in cell surface remodelling and the oxidative stress response
Microbiology 156, 2873 (2010); https://doi.org/10.1099/mic.0.037887-0
/content/journal/micro/10.1099/mic.0.037887-0
/content/journal/micro/10.1099/mic.0.037887-0
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