1887

Abstract

The growth of DSM 7145 on -erythritol is characterized by two stages: in the first stage, -erythritol is oxidized almost stoichiometrically to -erythrulose according to the Bertrand–Hudson rule. The second phase is distinguished from the first phase by a global metabolic change from membrane-bound -erythritol oxidation to -erythrulose assimilation with concomitant accumulation of acetic acid. The membrane-associated erythritol-oxidizing enzyme was found to be encoded by a gene homologous to known from other species of acetic acid bacteria. Disruption of this gene in the genome of DSM 7145 revealed that the membrane-bound polyol dehydrogenase not only oxidizes -erythritol but also has a broader substrate spectrum which includes C3–C6 polyols and -gluconate and supports growth on these substrates. Cultivation of DSM 7145 on different substrates indicated that expression of the polyol dehydrogenase was not regulated, implying that the production of biomass of to be used as whole-cell biocatalysts in the biotechnological conversion of -erythritol to -erythrulose, which is used as a tanning agent in the cosmetics industry, can be conveniently carried out with glucose as the growth substrate.

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2010-06-01
2019-12-07
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Alignment of the amino acid sequences of the SldB and SldA proteins of ATCC 621H (Gox0855) and DSM 7145. [PDF](35 KB)

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