1887

Abstract

Putative endolysin genes of bacteriophages CMP1 and CN77, which infect subsp. and subsp. , respectively, were cloned and expressed in . The His-tagged endolysin of CMP1 consists of 306 amino acids and has a calculated molecular mass of 34.8 kDa, while the His-tagged endolysin of CN77 has 290 amino acids with a molecular mass of 31.9 kDa. The proteins were purified and their bacteriolytic activity was demonstrated. The bacteriolytic activity of both enzymes showed a host range which was limited to the respective subspecies and did not affect other bacteria, even those closely related to . Due to the high specificity of the CMP1 and CN77 endolysins they may be useful tools for biocontrol of plant-pathogenic without affecting other bacteria in the soil.

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2010-08-01
2020-08-12
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References

  1. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J.. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res25:3389–3402
    [Google Scholar]
  2. Balogh B., Jones J. B.. 2003; Improved efficacy of newly formulated bacteriophages for management of bacterial spot on tomato. Plant Dis87:949–954
    [Google Scholar]
  3. Beilstein F., Dreiseikelmann B.. 2006; Bacteriophages of freshwater Brevundimonas vesicularis isolates. Res Microbiol157:213–219
    [Google Scholar]
  4. Cook F. D., Katznelson H.. 1960; Isolation of bacteriophages for the detection of Corynebacterium insidiosum, agent of bacterial wilt of alfalfa. Can J Microbiol6:121–126
    [Google Scholar]
  5. Davis M. J., Gillespie A. G. Jr, Vidaver A. K., Harris R. W.. 1984; Clavibacter: a new genus containing some phytopathogenic coryneform bacteria, including Clavibacter xyli subsp.xyli sp. nov., subsp. nov. and Clavibacterxyli subsp. cynodontis subsp. nov., pathogens that cause ratoon stunting disease of sugarcane and bermudagrass stunting disease. Int J Syst Bacteriol34:107–117
    [Google Scholar]
  6. Disqué-Kochem C., Dreiseikelmann B.. 1997; The cytoplasmic DNA-binding protein TraM binds to the inner membrane protein TraD in vitro. J Bacteriol179:6133–6137
    [Google Scholar]
  7. Eayre C. G., Concelmo D. E., Bartz J. A.. 1990; Control of soft rot Erwinias with bacteriophages. Phytopathology80:994
    [Google Scholar]
  8. Echandi E., Sun M.. 1973; Isolation and characterization of a bacteriophage for the identification of Corynebacterium michiganense. Phytopathology63:1401–1403
    [Google Scholar]
  9. Eichenlaub R., Gartemann K.-H., Burger A.. 2007; Clavibacter michiganensis, a group of Gram-positive pathogenic bacteria. In Plant-Associated Bacteria pp383–422 Edited by Gnanamanickam S. S.. Dordrecht: Springer;
  10. Evtushenko L. I., Dorofeeva L. V., Subbotin S. A., James R., Cole J. R., Tiedje J. M.. 2000; Leifsonia poae gen. nov., sp. nov., isolated from nematode galls on Poa annua, and reclassification of ‘Corynebacterium aquaticum ’ Leifson 1962 as Leifsonia aquatica (ex Leifson 1962) gen. nov., nom. rev., comb. nov. and Clavibacter xyli Davis et al. 1984 with two subspecies as Leifsonia xyli (Davis et al. 1984) gen. nov., comb. nov. Int J Syst Evol Microbiol 50:371–380
    [Google Scholar]
  11. Fischetti V. A.. 2008; Bacteriophage lysins as effective antibacterials. Curr Opin Microbiol11:393–400
    [Google Scholar]
  12. Garcia P., Garcia J. L., Garcia E., Sanchez-Puelles J. M., Lopez R.. 1990; Modular organization of the lytic enzymes of Streptococcus pneumoniae and its bacteriophages. Gene86:81–88
    [Google Scholar]
  13. Jones J. B., Jackson L. E., Balogh B., Obradovic A., Iriarte F. B., Momol M. T.. 2007; Bacteriophages for plant disease control. Annu Rev Phytopathol45:245–262
    [Google Scholar]
  14. Kleitman F., Barash I., Burger A., Iraki N., Falah Y., Sessa G., Weinthal D., Chalupowicz L., Gartemann K.-H.. other authors 2008; Characterization of a Clavibacter michiganensis subsp. michiganensis population in Israel. Eur J Plant Pathol121:463–475
    [Google Scholar]
  15. Krogh A., Larsson B., von Heijne G., Sonnhammer E. L. L.. 2001; Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes. J Mol Biol305:567–580
    [Google Scholar]
  16. Laemmli U. K.. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature227:680–685
    [Google Scholar]
  17. Laine M. J., Nakhei H., Dreier J., Lehtila K., Meletzus D., Eichenlaub R., Metzler M. C.. 1996; Stable transformation of the Gram-positive phytopathogenic bacterium Clavibacter michiganensis subsp. sepedonicus. Appl Environ Microbiol62:1500–1506
    [Google Scholar]
  18. Lanka E., Barth P. T.. 1981; Plasmid RP4 specifies a deoxyribonucleic acid primase involved in its conjugal transfer and maintenance. J Bacteriol148:769–781
    [Google Scholar]
  19. Lee I.-M., Batoszyk I. M., Gundersen-Rindal D. E., Davis R. E.. 1997; Phylogeny and classification of bacteria in the genera Clavibacter and Rathayibacter on the basis of 16S rRNA gene sequence analyses. Appl Environ Microbiol63:2631–2636
    [Google Scholar]
  20. Loessner M. J.. 2005; Bacteriophage endolysins – current state of research and applications. Curr Opin Microbiol8:480–487
    [Google Scholar]
  21. Loessner M. J., Wendlinger G., Scherer S.. 1995; Heterogeneous endolysins in Listeria monocytogenes bacteriophages: a new class of enzymes and evidence for conserved holin genes within siphoviral lysis cassettes. Mol Microbiol16:1231–1241
    [Google Scholar]
  22. Parisien A., Allain B., Zhang J., Mandeville R., Lan C. Q.. 2008; Novel alternatives to antibiotics: bacteriophages, bacterial cell wall hydrolases, and antimicrobial peptides. J Appl Microbiol104:1–13
    [Google Scholar]
  23. Park Y.-H., Suzuki K.-I., Yim D.-G., Lee K.-C., Kim E., Yoon J.-S., Kim S.-J., Kho Y.-H., Goodfellow M., Komogata K.. 1993; Suprageneric classification of peptidoglycan group B actinomycetes by nucleotide sequencing of 5S ribosomal RNA. Antonie Van Leeuwenhoek64:307–313
    [Google Scholar]
  24. Rawlings N. D., Morton F. R., Kok C. Y., Kong J., Barrett A. J.. 2008; MEROPS: the peptidase database. Nucleic Acids Res36: (Database issue), D320–D325
    [Google Scholar]
  25. Sasaki J., Chijimatsu M., Suzuki K.. 1998; Taxonomic significance of 2,4-diaminobutyric acid isomers in the cell wall peptidoglycan of actinomycetes and reclassification of Clavibacter toxicus as Rathayibacter toxicus comb. nov. Int J Syst Bacteriol48:403–410
    [Google Scholar]
  26. Schleifer K.-H., Kandler O.. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev36:407–477
    [Google Scholar]
  27. Schnabel E. L., Fernando W. G. D., Jackson L. L., Meyer M. P., Jones A. L.. 1998; Bacteriophages of Erwinia amylovora and their potential in biocontrol. Appl Environ Microbiol68:1122–1131
    [Google Scholar]
  28. Shirako Y., Vidaver A. K., Ackermann H. W.. 1986; Partial characterization of bacteriophages for Clavibacter michiganense subsp. nebraskense. Ann Phytopathol Soc Jpn52:793–800
    [Google Scholar]
  29. Strider D. L.. 1969; Bacterial Canker of Tomato Caused by Corynebacterium michiganense. A Literature Review and Bibliography. North Carolina Agric. Exp. Sta., Tech. Bullno: 193
    [Google Scholar]
  30. Sulakvelidze A., Barrow P.. 2005; Phage therapy in animals and agribusiness. In Bacteriophages: Biology and Applications pp335–380 Edited by Kutter E., Sulakvelidze A.. Boca Raton, FL: CRC Press;
  31. Sulakvelidze A., Kutter E.. 2005; Bacteriophage therapy in humans. In Bacteriophages: Biology and Applications pp381–436 Edited by Kutter E., Sulakvelidze A. Boca Raton, FL: CRC Press;
  32. Tanaka H., Negishi H., Maeda H.. 1990; Control of tobacco bacterial wilt by an avirulent strain of Pseudomonas solanacearum M4S and its bacteriophage. Ann Phytopathol Soc Jpn56:243–246
    [Google Scholar]
  33. Vidaver A. K., Gross D. C., Wysong D. S., Doupnik B. L.. 1981; Diversity of Corynebacterium nebraskense strains causing Goss's bacterial wilt and blight of corn. Plant Dis65:480–482
    [Google Scholar]
  34. White M. C., Decker A. M., Chaney R. L.. 1981; Metal complexation in xylem fluid. I. Chemical composition of tomato and soybean stem exudate. Plant Physiol67:292–300
    [Google Scholar]
  35. Young R., Bläsi U.. 1995; Holins: form and function in bacteriophage lysis. FEMS Microbiol Rev17:191–205
    [Google Scholar]
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