1887

Abstract

Putative endolysin genes of bacteriophages CMP1 and CN77, which infect subsp. and subsp. , respectively, were cloned and expressed in . The His-tagged endolysin of CMP1 consists of 306 amino acids and has a calculated molecular mass of 34.8 kDa, while the His-tagged endolysin of CN77 has 290 amino acids with a molecular mass of 31.9 kDa. The proteins were purified and their bacteriolytic activity was demonstrated. The bacteriolytic activity of both enzymes showed a host range which was limited to the respective subspecies and did not affect other bacteria, even those closely related to . Due to the high specificity of the CMP1 and CN77 endolysins they may be useful tools for biocontrol of plant-pathogenic without affecting other bacteria in the soil.

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2010-08-01
2019-10-20
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vol. , part 8, pp. 2366 - 2373

Lysis assay with CMP1 endolysin on different strains [PDF](55 KB)



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