1887

Abstract

mutations, which cause acidic phospholipid deficiency, repress transcription of the flagellar master operon , and thus impair flagellar formation and motility. The molecular mechanism of the strong repression of transcription in the mutant cells, however, has not yet been clarified. In order to shed light on this mechanism we isolated genes which, when supplied in multicopy, suppress the repression of , and found that three genes, , and were capable of suppression. Taking into account a previous report that represses production, the level of in the mutant was examined. We found that cells had a high level of and that introduction of a plasmid into cells did reduce the level. The cells exhibited a sharp increase in levels that can only be partially attributed to the slight increase in transcription; the largest part of the effect is due to a post-transcriptional accumulation of . GadW in multicopy exerts its effect by post-transcriptionally downregulating . YeaB and MetE in multicopy also exert their effect via . Disruption of caused an increase of the mRNA level, and induction from P- repressed the mRNA level. The strong repression of transcription in mutant cells is thus suggested to be caused by the accumulated .

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2010-06-01
2019-10-14
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