1887

Abstract

Cultures of ruminal bacteria known to metabolize unsaturated fatty acids were grown in medium containing 50 μg ml of geometric and positional isomers of conjugated linoleic acid (CLA) or 18 : 1 fatty acids and 37.4 % deuterium oxide to investigate the mechanisms responsible for fatty acid metabolism. JW11 converted 9,11-18 : 2 and 9,11-18 : 2 to -11-18 : 1 as the main product, labelled at C-9, and metabolized 10,12-18 : 2 to 10-18 : 1, labelled at C-13, and smaller amounts of -12-18 : 1 and -12-18 : 1. P-18 did not grow in the presence of 9,11-18 : 2 or 10,12-18 : 2, but grew in medium containing 9,11-18 : 2, forming 18 : 0. , a ruminal species that isomerizes linoleic acid to 10,12-18 : 2, did not metabolize CLA isomers further. metabolized small amounts of 10-18 : 1, 11-18 : 1 and 9-18 : 1, but the products formed were not detected. , on the other hand, carried out substantial conversion of 18 : 1 substrates to 18 : 0. hydrated 9-18 : 1 and 11-18 : 1 to 10-OH-18 : 0, which was further oxidized to yield 10-O-18 : 0. The deuterium enrichment in the intermediates formed during incubations with 9,11 geometric isomers of CLA was about half that of the products from 10,12 CLA and 18 : 1 isomers, suggesting that the reduction of 9,11 geometric isomers CLA by ruminal bacteria occurs via different mechanisms compared with the metabolism of other unsaturated fatty acids.

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2010-02-01
2020-07-02
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