1887

Abstract

The yeast-like fungus favours respiration as a mechanism of energy production, and thus depends heavily on mitochondrial function. Previous studies of a Δ mutant revealed reduced expression of the mitochondrial elongation factor and defects in glycerol utilization, consistent with mitochondrial dysfunction. In this study, we found that expression of in the Δ mutant suppressed the mitochondrial defects, including growth on respiration-dependent carbon sources and fluconazole resistance, associated with deletion. Tetracycline, an inhibitor of mitochondrial translation, was found to confer resistance to fluconazole in the wild-type and Δ mutant, whereas the fluconazole susceptibility of the -overexpressing strain was unaffected by tetracycline treatment. In the presence of fluconazole, the Δ mutant exhibited increased activation of the global transcriptional regulator Sre1. overexpression failed to alter cleavage of Sre1 in response to fluconazole in the Δ mutant, suggesting that repression in the Δ mutant is distal to Sre1, or that it occurs through an independent pathway.

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2010-08-01
2020-01-22
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