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Microbiology Society logo Microbiology

Volume 156, Issue 8

Overexpression of restores respiratory growth and fluconazole sensitivity to a Δ mutant Free

Abstract

The yeast-like fungus favours respiration as a mechanism of energy production, and thus depends heavily on mitochondrial function. Previous studies of a Δ mutant revealed reduced expression of the mitochondrial elongation factor and defects in glycerol utilization, consistent with mitochondrial dysfunction. In this study, we found that expression of in the Δ mutant suppressed the mitochondrial defects, including growth on respiration-dependent carbon sources and fluconazole resistance, associated with deletion. Tetracycline, an inhibitor of mitochondrial translation, was found to confer resistance to fluconazole in the wild-type and Δ mutant, whereas the fluconazole susceptibility of the -overexpressing strain was unaffected by tetracycline treatment. In the presence of fluconazole, the Δ mutant exhibited increased activation of the global transcriptional regulator Sre1. overexpression failed to alter cleavage of Sre1 in response to fluconazole in the Δ mutant, suggesting that repression in the Δ mutant is distal to Sre1, or that it occurs through an independent pathway.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): qRT-PCR, quantitative real-time PCR
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/content/journal/micro/10.1099/mic.0.035923-0
2010-08-01
2025-04-20
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/content/journal/micro/10.1099/mic.0.035923-0
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Overexpression of TUF1 restores respiratory growth and fluconazole sensitivity to a Cryptococcus neoformansvad1Δ mutant
Microbiology 156, 2558 (2010); https://doi.org/10.1099/mic.0.035923-0
/content/journal/micro/10.1099/mic.0.035923-0
/content/journal/micro/10.1099/mic.0.035923-0
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