1887

Abstract

Plasmid pBaSysBioII was constructed for high-throughput analysis of gene expression in . It is an integrative plasmid with a ligation-independent cloning (LIC) site, allowing the generation of transcriptional fusions with desired promoters. Integration is by a Campbell-type event and is non-mutagenic, placing the fusion at the homologous chromosomal locus. Using , , and promoters that are responsive to phosphate availability, growth rate and carbon source, we show that detailed profiles of promoter activity can be established, with responses to changing conditions being measurable within 1 min of the stimulus. This makes pBaSysBioII a highly versatile tool for real-time gene expression analysis in growing cells of

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2010-06-01
2019-10-22
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