1887

Abstract

species are currently the only known organisms with two types of genes encoding UDP-glucose/-galactose 4-epimerase, and . A strain deleted for exhibited a severe galactosylation defect and a decrease in activity and in UDP-galactose content when grown in glucose-rich medium (2 % glucose), indicating that Uge1p is a major UDP-glucose/-galactose 4-epimerase under these growth conditions. In contrast, was efficiently expressed and involved in galactosylation of cell-surface proteins in low-glucose medium (0.1 % glucose and 2 % glycerol), but not in galactose-containing medium. In a ΔΔ strain, the galactosylation defect was suppressed and UDP-galactose content restored to wild-type levels in galactose-containing medium. Disruption of , encoding galactose-1-phosphate uridylyltransferase, in the ΔΔ strain reversed suppression of the galactosylation defect and reduced levels of UDP-galactose, indicating that galactose is transported from the medium to the cytosol and is converted into UDP-galactose via galactose 1-phosphate by Gal7p in .

Loading

Article metrics loading...

/content/journal/micro/10.1099/mic.0.035279-0
2010-03-01
2020-08-09
Loading full text...

Full text loading...

/deliver/fulltext/micro/156/3/708.html?itemId=/content/journal/micro/10.1099/mic.0.035279-0&mimeType=html&fmt=ahah

References

  1. Alfa C., Fantes P., Hyams J., McLoed M., Warbrick E.. 1993; Experiments with Fission Yeast: a Laboratory Course Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
  2. Andreishcheva E. N., Kunkel J. P., Gemmill T. R., Trimble R. B.. 2004; Five genes involved in biosynthesis of the pyruvylated Gal β 1,3-epitope in Schizosaccharomyces pombe N-linked glycans. J Biol Chem279:35644–35655
    [Google Scholar]
  3. Basi G., Schmid E., Maundrell K.. 1993; TATA box mutations in the Schizosaccharomyces pombe nmt1 promoter affect transcription efficiency but not the transcription start point or thiamine repressibility. Gene123:131–136
    [Google Scholar]
  4. Brahma A., Bhattacharyya D.. 2004; UDP-galactose 4-epimerase from Kluyveromyces fragilis. Evidence for independent mutarotation site. Eur J Biochem271:58–68
    [Google Scholar]
  5. Chappell T. G., Hajibagheri M. A., Ayscough K., Pierce M., Warren G.. 1994; Localization of an α-1,2 galactosyltransferase activity to the Golgi apparatus of Schizosaccharomyces pombe. Mol Biol Cell5:519–528
    [Google Scholar]
  6. Dean N.. 1995; Yeast glycosylation mutants are sensitive to aminoglycosides. Proc Natl Acad Sci U S A92:1287–1291
    [Google Scholar]
  7. De Robichon-Szulmajster H.. 1958; Induction of enzymes of the galactose pathway in mutants of Saccharomyces cerevisiae. Science127:28–29
    [Google Scholar]
  8. Dibenedetto G., Cozzani I.. 1975; Nonspecific acid phosphatase from Schizosaccharomyces pombe. Purification and physical chemical properties. Biochemistry14:2847–2852
    [Google Scholar]
  9. Douglas H. C., Condie F.. 1954; The genetic control of galactose utilization in Saccharomyces. J Bacteriol68:662–670
    [Google Scholar]
  10. Fedor M. J., Kornberg R. D.. 1989; Upstream activation sequence-dependent alteration of chromatin structure and transcription activation of the yeast GAL1GAL10 genes. Mol Cell Biol9:1721–1732
    [Google Scholar]
  11. Forsburg S. L., Rhind N.. 2006; Basic methods for fission yeast. Yeast23:173–183
    [Google Scholar]
  12. Frey P. A.. 1996; The Leloir pathway: a mechanistic imperative for three enzymes to change the stereochemical configuration of a single carbon in galactose. FASEB J10:461–470
    [Google Scholar]
  13. Gemmill T. R., Trimble R. B.. 1996; Schizosaccharomyces pombe produces novel pyruvate-containing N-linked oligosaccharides. J Biol Chem271:25945–25949
    [Google Scholar]
  14. Gemmill T. R., Trimble R. B.. 1998; All pyruvylated galactose in Schizosaccharomyces pombe N-glycans is present in the terminal disaccharide,4,6- O-[( R)-(1-carboxyethylidine)]-Gal β 1,3Gal α1. Glycobiology 8:1087–1095
    [Google Scholar]
  15. Gemmill T. R., Trimble R. B.. 1999; Overview of N- and O-linked oligosaccharide structures found in various yeast species. Biochim Biophys Acta 1426;227–237
    [Google Scholar]
  16. Hashimoto H., Kikuchi Y., Nogi Y., Fukasawa T.. 1983; Regulation of expression of the galactose gene cluster in Saccharomyces cerevisiae. Isolation and characterization of the regulatory gene GAL4. Mol Gen Genet191:31–38
    [Google Scholar]
  17. Heiland S., Radovanovic N., Hofer M., Winderickx J., Lichtenberg H.. 2000; Multiple hexose transporters of Schizosaccharomyces pombe. J Bacteriol182:2153–2162
    [Google Scholar]
  18. Hoffman C. S.. 2005; Except in every detail: comparing and contrasting G-protein signaling in Saccharomyces cerevisiae and Schizosaccharomyces pombe. Eukaryot Cell4:495–503
    [Google Scholar]
  19. Holden H. M., Rayment I., Thoden J. B.. 2003; Structure and function of enzymes of the Leloir pathway for galactose metabolism. J Biol Chem278:43885–43888
    [Google Scholar]
  20. Huang K. M., Snider M. D.. 1995; Isolation of protein glycosylation mutants in the fission yeast Schizosaccharomyces pombe. Mol Biol Cell6:485–496
    [Google Scholar]
  21. Ikeda Y., Ohashi T., Tanaka N., Takegawa K.. 2009; Identification and characterization of a gene required for α1,2-mannose extension in the O-linked glycan synthesis pathway in Schizosaccharomyces pombe. FEMS Yeast Res9:115–125
    [Google Scholar]
  22. Johnston M.. 1999; Feasting, fasting and fermenting. Glucose sensing in yeast and other cells. Trends Genet15:29–33
    [Google Scholar]
  23. Keegan L., Gill G., Ptashne M.. 1986; Separation of DNA binding from the transcription-activating function of a eukaryotic regulatory protein. Science231:699–704
    [Google Scholar]
  24. Kig C., Turkel S., Temizkan G.. 2005; Isolation and characterization of glucose derepressed invertase mutants from Schizosaccharomyces pombe. Biosci Biotechnol Biochem69:2475–2478
    [Google Scholar]
  25. Lohr D., Venkov P., Zlatanova J.. 1995; Transcriptional regulation in the yeast GAL gene family: a complex genetic network. FASEB J9:777–787
    [Google Scholar]
  26. Majumdar S., Ghatak J., Mukherji S., Bhattacharjee H., Bhaduri A.. 2004; UDPgalactose 4-epimerase from Saccharomyces cerevisiae. A bifunctional enzyme with aldose 1-epimerase activity. Eur J Biochem271:753–759
    [Google Scholar]
  27. Manners D. J., Meyer M. T.. 1977; The molecular structures of some glucans from the cell walls of Schizosaccharomyces pombe. Carbohydr Res57:189–203
    [Google Scholar]
  28. Maundrell K.. 1990; nmt1 of fission yeast. A highly transcribed gene completely repressed by thiamine. J Biol Chem265:10857–10864
    [Google Scholar]
  29. Maundrell K.. 1993; Thiamine-repressible expression vectors pREP and pRIP for fission yeast. Gene123:127–130
    [Google Scholar]
  30. Moreno S., Ruiz T., Sanchez Y., Villanueva J. R., Rodriguez L.. 1985; Subcellular localization and glycoprotein nature of the invertase from the fission yeast Schizosaccharomyces pombe. Arch Microbiol142:370–374
    [Google Scholar]
  31. Moreno S., Klar A., Nurse P.. 1991; Molecular genetic analysis of fission yeast Schizosaccharomyces pombe. Methods Enzymol194:795–823
    [Google Scholar]
  32. Morita T., Takegawa K.. 2004; A simple and efficient procedure for transformation of Schizosaccharomyces pombe. Yeast21:613–617
    [Google Scholar]
  33. Nakamura T., Nakamura-Kubo M., Hirata A., Shimoda C.. 2001; The Schizosaccharomyces pombe spo3+ gene is required for assembly of the forespore membrane and genetically interacts with psy1+-encoding syntaxin-like protein. Mol Biol Cell12:3955–3972
    [Google Scholar]
  34. Neely L. A., Hoffman C. S.. 2000; Protein kinase A and mitogen-activated protein kinase pathways antagonistically regulate fission yeast fbp1 transcription by employing different modes of action at two upstream activation sites. Mol Cell Biol20:6426–6434
    [Google Scholar]
  35. Nehlin J. O., Carlberg M., Ronne H.. 1989; Yeast galactose permease is related to yeast and mammalian glucose transporters. Gene85:313–319
    [Google Scholar]
  36. Ohashi T., Ikeda Y., Tanaka N., Nakakita S., Natsuka S., Giga-Hama Y., Takegawa K.. 2009; The och1 mutant of Schizosaccharomyces pombe produces galactosylated core structures of N-linked oligosaccharides. Biosci Biotechnol Biochem73:407–414
    [Google Scholar]
  37. Okazaki K., Okazaki N., Kume K., Jinno S., Tanaka K., Okayama H.. 1990; High-frequency transformation method and library transducing vectors for cloning mammalian cDNAs by trans-complementation of Schizosaccharomyces pombe. Nucleic Acids Res18:6485–6489
    [Google Scholar]
  38. Peng G., Hopper J. E.. 2002; Gene activation by interaction of an inhibitor with a cytoplasmic signaling protein. Proc Natl Acad Sci U S A99:8548–8553
    [Google Scholar]
  39. Platt A., Reece R. J.. 1998; The yeast galactose genetic switch is mediated by the formation of a Gal4p-Gal80p-Gal3p complex. EMBO J17:4086–4091
    [Google Scholar]
  40. Rolland F., Winderickx J., Thevelein J. M.. 2001; Glucose-sensing mechanisms in eukaryotic cells. Trends Biochem Sci26:310–317
    [Google Scholar]
  41. Rolland F., Winderickx J., Thevelein J. M.. 2002; Glucose-sensing and -signalling mechanisms in yeast. FEMS Yeast Res2:183–201
    [Google Scholar]
  42. Sambrook J., Fritsch E., Maniatis T.. 1989; Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
  43. Schweingruber M. E., Fluri R., Maundrell K., Schweingruber A. M., Dumermuth E.. 1986; Identification and characterization of thiamin repressible acid phosphatase in yeast. J Biol Chem261:15877–15882
    [Google Scholar]
  44. Scott A., Timson D. J.. 2007; Characterization of the Saccharomyces cerevisiae galactose mutarotase/UDP-galactose4-epimerase protein, Gal10p. FEMS Yeast Res7:366–371
    [Google Scholar]
  45. Suga M., Hatakeyama T.. 2001; High efficiency transformation of Schizosaccharomyces pombe pretreated with thiol compounds by electroporation. Yeast18:1015–1021
    [Google Scholar]
  46. Suga M., Isobe M., Hatakeyama T.. 2000; Cryopreservation of competent intact yeast cells for efficient electroporation. Yeast16:889–896
    [Google Scholar]
  47. Tabuchi M., Tanaka N., Iwahara S., Takegawa K.. 1997; The Schizosaccharomyces pombe gms1+ gene encodes an UDP-galactose transporter homologue required for protein galactosylation. Biochem Biophys Res Commun232:121–125
    [Google Scholar]
  48. Takegawa K., Tanaka N., Tabuchi M., Iwahara S.. 1996; Isolation and characterization of a glycosylation mutant from Schizosaccharomyces pombe. Biosci Biotechnol Biochem60:1156–1159
    [Google Scholar]
  49. Tanaka N., Takegawa K.. 2001; Functional characterization of Gms1p/UDP-galactose transporter in Schizosaccharomyces pombe. Yeast18:745–757
    [Google Scholar]
  50. Tanaka N., Ohuchi N., Mukai Y., Osaka Y., Ohtani Y., Tabuchi M., Bhuiyan M. S., Fukui H., Harashima S., Takegawa K.. 1998; Isolation and characterization of an invertase and its repressor genes from Schizosaccharomyces pombe. Biochem Biophys Res Commun245:246–253
    [Google Scholar]
  51. Tanaka N., Awai A., Bhuiyan M. S., Fujita K., Fukui H., Takegawa K.. 1999; Cell surface galactosylation is essential for nonsexual flocculation in Schizosaccharomyces pombe. J Bacteriol181:1356–1359
    [Google Scholar]
  52. Tanaka N., Konomi M., Osumi M., Takegawa K.. 2001; Characterization of a Schizosaccharomyces pombe mutant deficient in UDP-galactose transport activity. Yeast18:903–914
    [Google Scholar]
  53. Thoden J. B., Holden H. M.. 2005; The molecular architecture of galactose mutarotase/UDP-galactose4-epimerase from Saccharomyces cerevisiae. J Biol Chem280:21900–21907
    [Google Scholar]
  54. Tschopp J. F., Emr S. D., Field C., Schekman R.. 1986; GAL2 codes for a membrane-bound subunit of the galactose permease in Saccharomyces cerevisiae. J Bacteriol166:313–318
    [Google Scholar]
  55. Umeda K., Yoko-o T., Nakayama K., Suzuki T., Jigami Y.. 2000; Schizosaccharomyces pombe gmd3+/ alg11+ is a functional homologue of Saccharomyces cerevisiae ALG11 which is involved in N-linked oligosaccharide synthesis. Yeast16:1261–1271
    [Google Scholar]
  56. Yoko-o T., Roy S. K., Jigami Y.. 1998; Differences in in vivo acceptor specificity of two galactosyltransferases, the gmh3+ and gma12+ gene products from Schizosaccharomyces pombe. Eur J Biochem257:630–637
    [Google Scholar]
  57. Yoko-o T., Tsukahara K., Watanabe T., Hata-Sugi N., Yoshimatsu K., Nagasu T., Jigami Y.. 2001; Schizosaccharomyces pombe och1+ encodes α-1,6-mannosyltransferase that is involved in outer chain elongation of N-linked oligosaccharides. FEBS Lett489:75–80
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/mic.0.035279-0
Loading
/content/journal/micro/10.1099/mic.0.035279-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error