%0 Journal Article %A Tayler, Amy E. %A Ayala, Juan A. %A Niumsup, Pannika %A Westphal, Katrin %A Baker, Jenny A. %A Zhang, Lufei %A Walsh, Timothy R. %A Wiedemann, Bernd %A Bennett, Peter M. %A Avison, Matthew B. %T Induction of β-lactamase production in Aeromonas hydrophila is responsive to β-lactam-mediated changes in peptidoglycan composition %D 2010 %J Microbiology, %V 156 %N 8 %P 2327-2335 %@ 1465-2080 %R https://doi.org/10.1099/mic.0.035220-0 %K PBP, penicillin-binding protein %K TCS, two-component system %I Microbiology Society, %X We have studied the mechanism by which β-lactam challenge leads to β-lactamase induction in Aeromonas hydrophila through transposon-insertion mutagenesis. Disruption of the dd-carboxypeptidases/endopeptidases, penicillin-binding protein 4 or BlrY leads to elevated monomer-disaccharide-pentapeptide levels in A. hydrophila peptidoglycan and concomitant overproduction of β-lactamase through activation of the BlrAB two-component regulatory system. During β-lactam challenge, monomer-disaccharide-pentapeptide levels increase proportionately with β-lactamase production and β-lactamase induction is inhibited by vancomycin, which binds muro-pentapeptides. Taken together, these data strongly suggest that the Aeromonas spp. β-lactamase regulatory sensor kinase, BlrB, responds to the concentration of monomer-disaccharide-pentapeptide in peptidoglycan. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.035220-0