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Volume 156, Issue 7

Ribulose-1,5-bisphosphate carboxylase/oxygenase genes as a functional marker for chemolithoautotrophic halophilic sulfur-oxidizing bacteria in hypersaline habitats Free

Abstract

The presence and diversity of the genes encoding the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) (a key enzyme of the Calvin–Benson cycle of autotrophic CO assimilation) were investigated in pure cultures of seven genera of halophilic chemolithoautotrophic sulfur-oxidizing bacteria (SOB) and in sediments from a hypersaline lake in which such bacteria have been recently discovered. All of the halophilic SOB strains (with the exception of ) possessed the gene encoding RuBisCO form I, while the gene encoding RuBisCO form II was detected only in some of the pure cultures. The general topologies of the CbbL/CbbM trees and the 16S rRNA gene tree were different, but both markers showed that the halophilic SOB genera formed independent lineages in the Gammaproteobacteria. In some cases, such as with several strains of the genus and with , the clustering was incongruent with the positions of these strains on the ribosomal tree. In the tree, the clustering of and strains was incongruent with their branching in both and 16S rRNA gene trees. and genes related to those found in the analysed halophilic SOB were also detected in a sediment from a hypersaline lake in Kulunda Steppe (Russia). Most of the and genes belonged to members of the genus . In the clone library, sequences related to those of and were detected as minor components. Some of the environmental sequences belonged to as yet unknown phylotypes, representing deep lineages of halophilic autotrophs.

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  • Accepted:
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  • Published Online:
Keyword(s): RuBisCO, ribulose-1,5-bisphosphate carboxylase/oxygenase and SOB, sulfur-oxidizing bacteria
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2010-07-01
2024-04-19
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Ribulose-1,5-bisphosphate carboxylase/oxygenase genes as a functional marker for chemolithoautotrophic halophilic sulfur-oxidizing bacteria in hypersaline habitats
Microbiology 156, 2016 (2010); https://doi.org/10.1099/mic.0.034603-0
/content/journal/micro/10.1099/mic.0.034603-0
/content/journal/micro/10.1099/mic.0.034603-0
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