1887

Abstract

sp. strain JC1 is able to grow on methanol as a sole source of carbon and energy using methanol : ′-dimethyl-4-nitrosoaniline oxidoreductase (MDO) as a key enzyme for primary methanol oxidation. Purified MDO oxidizes ethanol and formaldehyde as well as methanol. The sp. strain JC1 gene for MDO () was cloned, sequenced, and determined to have an open reading frame of 1272 bp. Northern blot and promoter analysis revealed that transcription was induced in cells grown in the presence of methanol. Northern blotting together with RT-PCR also showed that the gene was transcribed as monocistronic mRNA. Primer extension analysis revealed that the transcriptional start site of the gene is located 21 bp upstream of the start codon. An -deficient mutant of sp. strain JC1 did not grow with methanol as a sole source of carbon and energy.

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2010-02-01
2019-10-22
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