RT Journal Article SR Electronic(1) A1 Kurthkoti, Krishna A1 Srinath, Thiruneelakantan A1 Kumar, Pradeep A1 Malshetty, Vidyasagar S. A1 Sang, Pau Biak A1 Jain, Ruchi A1 Manjunath, Ramanathapuram A1 Varshney, UmeshYR 2010 T1 A distinct physiological role of MutY in mutation prevention in mycobacteria JF Microbiology, VO 156 IS 1 SP 88 OP 93 DO https://doi.org/10.1099/mic.0.033621-0 PB Microbiology Society, SN 1465-2080, AB Oxidative damage to DNA results in the occurrence of 7,8-dihydro-8-oxoguanine (8-oxoG) in the genome. In eubacteria, repair of such damage is initiated by two major base-excision repair enzymes, MutM and MutY. We generated a MutY-deficient strain of Mycobacterium smegmatis to investigate the role of this enzyme in DNA repair. The MutY deficiency in M. smegmatis did not result in either a noteworthy susceptibility to oxidative stress or an increase in the mutation rate. However, rifampicin-resistant isolates of the MutY-deficient strain showed distinct mutations in the rifampicin-resistance-determining region of rpoB. Besides the expected C to A (or G to T) mutations, an increase in A to C (or T to G) mutations was also observed. Biochemical characterization of mycobacterial MutY (M. smegmatis and M. tuberculosis) revealed an expected excision of A opposite 8-oxoG in DNA. Additionally, excision of G and T opposite 8-oxoG was detected. MutY formed complexes with DNA containing 8-oxoG : A, 8-oxoG : G or 8-oxoG : T but not 8-oxoG : C pairs. Primer extension reactions in cell-free extracts of M. smegmatis suggested error-prone incorporation of nucleotides into the DNA. Based on these observations, we discuss the physiological role of MutY in specific mutation prevention in mycobacteria., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.033621-0